缢蛏酶解产物清除羟自由基能力的研究

The ·OH free radical scavenging activities of hydrolysate from Sinonovacula constricta

研究利用水解位点较多的中性蛋白酶对缢蛏蛋白进行水解,以酶解产物(4mg/m L)对羟自由基的清除率为指标,对酶解工艺参数进行研究,通过正交试验优化酶解工艺,得出抗氧化活性最强的酶解工艺参数为:pH=7、料液比(m:V)为1:3.5、酶添加量1200 U/g、水解温度为40℃、水解时间为2h,在此参数下,羟自由基的清除率可达(76.01±0.3)%。

This study used neutral protease which has more hydrolysis of locus to hydrolyze protein of Sinonovacula constricta.The enzymatic hydrolysis process parameter were studied by useing the index of enzymatic hydrolysis product（4 mg/m L）of hydroxyl free radical clearance rate.It optimize the digestion process through Orthogonal test, It concluded that the strongest antioxidant activity of enzyme solution process parameters as follows p H=7, the adding amount was 1200 U/g, the feed liquid ratio（m：V） was 1：3.5, the hydrolysis temperature was 40 ℃, and the hydrolysis time was 2 h, under the technological conditions of hydroxyl free radical clearance rate can reach（76.01±0.3）%.