外周血microRNA在抑郁症患者中诊断价值的研究

Diagnostic value of microRNAs in peripheral venous blood for patients with depressive disorder

目的 探究外周血miRNA对抑郁症的诊断价值.方法 用RT-qPCR技术验证抑郁症患者外周血存在差异表达的miRNA在非特异性精神发育迟滞患者中的表达水平;采用ROC曲线分析比较在非特异性精神发育迟滞中不存在差异表达的miRNA在抑郁症、非特异性精神发育迟滞中的敏感度、特异度.结果 miR-1972,miR-26b,miR-4485,miR-4498,miR-4743在抑郁症病例组和健康对照组中表达上调且差异有统计学意义（P＜0.05）;其中miR-4485、miR-4743在非特异性精神发育迟滞病例组和健康对照组中表达上调且差异有统计学意义（P＜0.05）,miR-26b在非特异性精神发育迟滞病例组和健康对照组中表达无统计学意义（P＞0.05）.miR-26b在抑郁症病例组、健康对照组ROC曲线显示,其作为诊断指标的敏感度和特异度分别是0.609、0.664,曲线下面积为0.614（P=0.021）.miR-26b在抑郁症和非特异性精神发育迟滞患者ROC曲线显示,敏感度和特异度是0.784、0.471,曲线下面积为0.643（P=0.003）.结论 miR-26b可能对抑郁症的诊断具有重要意义,抑郁症、非特异性精神发育迟滞可能具有一定的共病特点,其具体机制尚有待进一步探讨.

Objective To investigate the diagnostic value of microRNA （miRNA） in peripheral venous blood for depressive disorder.Methods Real-time fluorescence quantitative PCR （RT-qPCR） was used to verify expression level of miRNAs in peripheral blood of non-specific mental retardation children,which were aberrantly expressed in depressive disorder patients,and then Receiver Operating Characteristics （ROC）curve was employed to confirm the sensitivity and specificity of abnormal miRNA expression in depressive disorder and non-specific mental retardation.Results MiR-1972,miR-26b,miR-4485,miR-4498 and miR-4743 were upregulated significantly in case group of depressive disorder（P〈0.05）,meanwhile miR-4485 and miR-4743 in aforementioned 5 miRNAs also upregulated significandy in patients of non-specific mental retardation（P〈0.05）,but miR-26b showed no significant difference between case group of non-specific mental retardation and the control group （P〉0.05）.The ROC curve of miR-26b in depressive disorder patients and their control group showed that the sensitivity and specificity were 0.609,0.664 respectively,and the area square under the curve was 0.614（P=0.021）.The ROC curve of miR-26b in patients of depressive disorder and non-specific mental retardation indicated that the sensitivity and specificity were 0.784 and 0.471,and area square under the curve was 0.643 （P=0.003）.Conclusion miR-26b probably have diagnostic value for depressive disorder,which may comorbidity with non-specific mental retardation.But genetic and psychosocial mechanism of comorbidity still needs further exploration.