给予外源性牛磺酸对HIBD幼鼠脑内氨基酸含量影响的初步研究

Influence of exogenous taurine addition in amino acid content in brains of hypoxic-ischemic brain injury rats during lactation

目的初步探讨给予外源性牛磺酸（TAU）对缺氧缺血性脑损伤（HIBD）模型幼鼠脑内氨基酸含量的影响。方法将36只7d龄SD大鼠按随机数字表法分为假手术组、对照组、TAU治疗组，每组12只，后2组参照Rice—Vannucci法构建HIBD模型。TAU治疗组在母乳喂养的基础上，于固定时间点添加300mg／（kg·d）TAU管饲，假手术组及对照组给予等体积生理盐水。采用高效液相色谱法分别检测术后48h及14d各组幼鼠脑内天冬氨酸（ASP）、谷氨酸（GLUl、甘氨酸（6LY）、TAU、γ-氨基丁酸（GABA）含量。结果术后48hTAU治疗组幼鼠损伤侧大脑皮层组织TAU及兴奋性氨基酸ASP、GLU含量与对照组相比显著升高，差异均有统计学意义（P〈0．05）；抑制性氨基酸GABA、GLY含量也升高，但差异无统计学意义（P〉0．05）；同时，GLU／TAU比例减小，与对照组相比差异有统计学意义（P〈0．05）。术后14dTAU治疗组幼鼠损伤侧大脑皮层组织TAU、GABA、GLY含量显著升高，GLU含量显著下降，ASP／GLY、GLU／GLY、GLU／TAU比例显著降低，与对照组比较差异均有统计学意义（P〈0．05）。结论给予外源性TAU可提高HIBD幼鼠脑内TAU及GABA、GLY含量，调节主要兴奋性／抑制性氨基酸比例。

Objective To investigate the effect of exogenous taurine （TAU） on amino acid content in the brains of rats suffered hypoxic-ischemic brain injury （HIBD）. Methods The 7-day-old SD rats were randomly divided into sham-operated group, control group and TAU treatment group. HIBD models in the later two groups were established following Rice-Vannucci method. On the basis of breastfeeding, TAU at 300 mg/（kg, d） via tube feeding to the TAU treatment group and an equal volume saline solution to the sham-operated group and control group were given. The aspartic acid （ASP）, glutamic acid （GLY）, glycine （GLY）, TAU and ~/- aminobutyric acid （GABA） contents in the rat brains were measured by high-performance liquid chromatography at 48 h and 14 d after HIBD. Results At 48 h after HIBD, the TAU, ASP and GLU contents in the TAU treatment group were significantly increased as compared with those in the control group （P〈0.05）; the GLU/TAU ratio in the TAU treatment group was significantly decreased as compared with that in the control group （P〈0.05）. Fourteen d after HIBD, the TAU, GABA and GLY contents were significantly increased, the GLU content was significantly decreased, and the ASP/GLY, GLU/GLY and GLU/TAU ratios were significantly deceased in the TAU treatment group as compared with those in the control group （P〈0.05）. Conclusion Exogenous TAU addition during lactation of rats suffered HIBD may improve contents of inhibitory amino acids as TAU, GABA and GLY, and modulate the ratio of main excitatory/inhibitory amino acids neurotransmitters.