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玉米锌指蛋白基因ZmAN11的序列分析及在非生物胁迫下的表达研究

Sequence Analysis of Maize Zinc- Finger Protein Gene Zm AN11 and Its Expression Status under Abiotic Stress
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摘要 对玉米锌指蛋白基因Zm AN11进行序列分析,结果表明Zm AN11开放阅读框长度为510 bp,编码一个含169个氨基酸的蛋白,预测分子量为18.06 k D,等电点是8.48。Zm AN11与水稻Osi SAP8同源性最高。在非生物胁迫下的表达研究发现,在盐、冷和热激胁迫下,Zm AN11表达量上调;而在干旱胁迫下,Zm AN11表达量下调。器官特异性分析发现,该基因在玉米叶片及胚芽鞘中表达量较高。Zm AN11基因的序列分析及表达研究为进一步研究其生物学功能和应用奠定了基础。 The sequence analysis of maize zinc- finger protein gene Zm AN11 showed that the ORF was510 bp,encoding a polypeptide with 169 amino acid residues. Its molecular mass was 18. 06 k D and the p I was 8. 48. Zm AN11 had the highest homology with Osi SAP8. The results of real- time quantitative fluorescence PCR showed that the expression level of Zm AN11 increased under Na Cl,cold and heat- shock stresses,but decreased under drought stress. Organ specificity analysis showed that the expression level of Zm AN11 was higher in leaves and coleoptiles. The sequence and expression analyses of Zm AN11 gene laid foundations for further study of its biological functions and practical application.
作者 宣宁 柳絮 张华 杨永义 姚方印
机构地区 山东省农业科学院生物技术研究中心
出处 《山东农业科学》 2015年第11期1-4,11,共5页 Shandong Agricultural Sciences
基金 国家自然科学基金项目(31171529) 山东省优秀中青年科学家科研奖励基金项目(BS2012SW019) 山东省农业科学院科技创新重点项目(2014CXZ10-2)
关键词 玉米 锌指蛋白 非生物胁迫 表达状况 Maize Zinc-finger protein Abiotic stress Expression status
分类号 S513 [农业科学—作物学] Q786 [生物学—分子生物学]
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参考文献24

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山东农业科学
2015年 第11期

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