高产酸乳酸菌的分离鉴定及生物学特性研究

Isolation and Identification of High Yield Lactic Acid Bacteria

采用改良乳酸菌选择性培养基从豆清发酵液中分离纯化出3株菌落形态各异的乳酸菌,对这3种菌发酵过程中p H及产酸量进行监测,筛选出一株产酸较多的优势菌株(编号LDS201)。结果表明:该菌落呈圆形,乳白色,边缘整齐,不透明,革兰氏染色阳性,菌体呈短杆状,单生或呈短链;经生理生化和16S rRNA基因序列分析鉴定该菌株为产酸解淀粉乳杆菌。

Using the modified lactic acid bacteria selective medium from tofu acid,3 strains with different colony morphology of lactic acid bacteria were separated. Monitoring of the 3 kinds of bacteria in the fermentation process of p H and acid production,the dominant strain of producing acid was selected（ No. LDS201）. The results showed that the colonies were round,milky white,the edge of the neat,opaque,gram positive,the bacteria were short,single or in a short chain; According to physiologicalbiochemical characteristics and 16 S rRNA gene sequence analysis,LDS201 was identified as Lactobacillus amylolyticus.