全氟辛烷磺酸对斑马鱼生理生化指标的影响研究

Effects of PFOS on the physiological and biochemical indices of Danio rerio

为了探讨全氟辛烷磺酸（PFOS）对鱼类的毒性效应,参考PFOS对斑马鱼（Danio rerio）的96 h-LC50值,设置了0、1、5和10 mg/L 4个浓度组,采用静水式实验测定了PFOS对斑马鱼头部碱性磷酸酶（ALP）、乳酸脱氢酶（LDH）和乙酰胆碱酯酶（AchE）,内脏团中ALP、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和丙二醛（MDA）含量以及肌肉中ALP、SOD、MDA含量。结果表明：斑马鱼头部ALP和AchE活力会在实验初期短时间内受到抑制,之后逐渐恢复;中低浓度PFOS（1 mg/L和5mg/L）胁迫会使得头部LDH活力显著升高。内脏团中ALP活力受到显著诱导,并与PFOS浓度呈一定的剂量-效应关系;SOD和CAT活性则在0.5~1 h受到显著诱导,之后有所下降,但并未受到显著抑制;MDA含量在PFOS胁迫下显著升高。肌肉中的ALP活力受到显著抑制（P〈0.05）,SOD活力及MDA含量受PFOS影响不显著。

Referring to the 96 h-LC50 values of Perfluorooctane sulfonate （PFOS） on Danio rerio, four groups with concentration of 0, 1,5 and 10 mg/L were set to explore the toxic effect of PFOS on fish. Indices of the physiological and biochemical parameters, such as the activities of alkaline phosphatase （ALP）, lactate dehydrogenase（ LDH）, and acetylcholinesterase （AchE） in the fish head, the superoxide dismutase （SOD）, catalase（CAT）, malondialdehyde （MDA） and ALP in the fish visceral were analyzed. ALP, SOD and MDA in the muscle of Danio rerio were also determined. The results showed that the activities of ALP and AchE in the head would be inhibited at the beginning of the experiment, then recover gradually. PFOS with mediumlow concentration （ 1 mg/L and 5 rag/L） induced LDH activity in the head to rise remarkably. ALP in the visceral mass was significantly induced and showed dose-effect relationship with PFOS concentration. After being significantly induced at 0.5 - 1 h, the activity of SOD and CAT declined later, but no significant inhibition was found. The content of MDA was significantly induced. In the muscle, the activity of ALP was significantly inhibited by PFOS（P 〈0.05）, but SOD activitv and MDA content were not affected_