匹诺塞林对局灶性脑缺血再灌注大鼠脑组织急性损伤的神经保护作用

Pinocembrin prevented brain acute injury induced by focal cerebral ischemia-reperfusion

目的:探讨并确证匹诺塞林对脑缺血再灌注急性损伤的神经保护作用,进一步为匹诺塞林治疗缺血性脑卒中的临床应用提供依据。方法:SD大鼠50只,随机均分为假手术组、模型组、匹诺塞林1 mg/kg组、匹诺塞林3 mg/kg组、匹诺塞林10 mg/kg组,其中模型组及匹诺塞林各给药组采用大脑中动脉阻塞法(middle cerebral artery ocelusion,MCAO)建立脑缺血再灌注模型,分别与大脑中动脉阻塞2h再灌注同时给药,6h后取血并断头取脑,制作病理切片。HE染色观察额顶叶皮层(缺血半暗带)和纹状体病理变化,尼氏染色观察海马CA1区形态变化。酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)检测大鼠血清神经元特异性烯醇化酶(neuronal specific enolase,NSE)及S100-β蛋白水平。结果:匹诺塞林能够改善大鼠急性局灶性脑缺血再灌注的脑组织皮层、纹状体和海马神经元的形态,降低血清中NSE和S100-β蛋白的水平(P<0.05或P<0.01)。结论:匹诺塞林能减轻缺血再灌注造成的脑组织急性损伤,具有神经保护作用。

AIM：The aim of this work was to verify neuroprotective and properties of Pinocembrin,then provide a reference for further analysis of its mechanism on ischemic stroke.METHODS：The study was carried out on 50 male Sprague-Dawley rats,weighing 260- 300 g,which were divided into five groups：（i） control（n = 10）,（ⅱ） I/R（n= 10）,（ⅲ） I/R ＋ Pinocembrin 1 mg/kg（n =10）;（ⅳ） I/R ＋ Pinocembrin 3 mg/kg（n = 10）;（v） I/R ＋ Pinocembrin 10 mg/kg（n = 10）.Focal cerebral ischemia/reperfusion rats were induced by middle cerebral artery occlusion（MCAO） for 2 h followed by 6 h reperfusion.Pinocembrin was administered in doses 1 mg/kg,3 mg/kg,10 mg/kg respectively at the same time of onset of reperfusion.Then the blood NSE and S-100β were determined in addition to observe the pathological change of hippocampus,penumbra cortex,and corpus striatum respectively.RESULTS：Pinocembrin-treatment（3mg/kg or 10 mg/kg） significantly suppressed the levels of blood NSE and S-100β,and improved the morphology of brain cortex,striatum and hippocampus of acute focal cerebral ischemia reperfusion（10mg/kg）.CONCLUSION：Pinocembrin has neuroprotective effects to prevent brain ischemia/reperfusion acute injury.