加兰他敏减轻脓毒症炎症反应作用的研究

Experimental study on effect of galanthamine reducing inflammatory reaction in sepsis

目的:研究中枢性胆碱醋酶抑制剂加兰他敏对脓毒症炎症反应的抑制作用。方法:采用改良的盲肠结扎穿孔(CLP)方法制备脓毒症模型。雄性SD大鼠50只随机分为5组,假手术组(SO组)、脓毒症组(SEP组)、加兰他敏处理组(Gal组)、阿托品处理组(Atr组)、加兰他敏+阿托品处理组(Gal+Atr组)。每组用药后6h后处死,抽取动脉血测IL-6、IL-1β、乳酸(Lac)。取左下肺和肝左叶组织,HE染色在光镜下观察肺脏和肝脏组织病理学改变。结果:与SO组比较,SEP组IL-6、IL-1β、乳酸的浓度明显提高(P<0.05),肝肺有明显的炎性病理改变;与SEP组比较,Atr组IL-6、IL-1β、乳酸的浓度更加提高(P<0.05),肝肺炎性病理改变更加明显;与SEP组比较,Gal组IL-6、IL-1β、乳酸的浓度明显降低(P<0.05),肝肺的炎性病理改变明显改善;而阿托品可抵消加兰他敏的作用。结论:加兰他敏可能通过胆碱能抗炎通路减轻脓毒症的炎症反应。

AIM：To study the effect of brain cholinesterase inhibitors galanthamine on anti-inflammatory response in sepsis.METHODS：50Male SD rats were randomly divided into sham operated（SO） group,sepsis（SEP） group,galanthamine（Gal） group,atropine（Atr） group,galanthamine ＋ atropine（Gal ＋ Atr） group.Sepsis model was produced by cecal ligation and puncture（CLP）.After 6 hours,IL-6,IL-1β and arterial blood lactic levels in blood were determined,and the tissues of lung and liver were harvested for histopathological analysis.RESULTS：Compared with SO group,IL-6,IL-1β and lactic levels in blood were increased（P〈0.05） and there were obvious pathologic changes in the lung and liver tissues in SEP group.Compared with SEP group,IL-6,IL-1βand lactic levels in blood were all increased（P〈0.05） and there were more pathologic changes in the lung and liver tissues in Atr group.Compared with SEP group,IL-6,IL-1β and lactic levels in blood were all decreased（P〈0.05） and the lung and liver tissue pathologic changes were slighter in Gal group.Atropine would offset such effects.CONCLUSION：Galantamine might reduce the inflammation of sepsis by the cholinergic anti-inflammatory pathway.