补饲植物雌激素对泌乳期伊犁母马血液生化指标及抗氧化力的影响

Effects of Phytooestrogens Supplementation on Blood Biochemical Indexes and Antioxidant Capacity of Lactating Yili Mare

试验旨在研究补饲植物雌激素对泌乳期伊犁母马血液生化指标及抗氧化能力的影响。根据年龄、胎次、泌乳月龄相近的原则选择泌乳期伊犁母马24匹,随机分为4组,分别为对照组、试验Ⅰ组、试验Ⅱ组和试验Ⅲ组。对照组饲喂基础日粮,试验Ⅰ、Ⅱ、Ⅲ组在饲喂基础日粮的基础上分别补饲0.7g大豆异黄酮、0.7g染料木素、0.35g大豆异黄酮和0.35g染料木素。预试期10d,正试期80d,分别于试验的第0、20、40、60、80天颈静脉采集血液样品,3 500r/min离心15min,分离血浆,-20℃保存备用。结果表明,与对照组相比,补饲植物雌激素对泌乳期伊犁母马血液中总蛋白、白蛋白含量和谷胱甘肽过氧化物酶、超氧化物歧化酶、过氧化氢酶活性的影响均不显著(P>0.05);但试验组过氧化氢酶活性、总抗氧化能力均有不同程度的提高;试验Ⅱ组血液中球蛋白、尿素氮含量显著高于对照组和试验Ⅰ组(P<0.05),其余各组间差异均不显著(P>0.05);与对照组相比,试验Ⅰ、Ⅱ组血液中丙二醛含量均呈降低趋势,且试验Ⅰ组达到显著水平(P<0.05)。因此,补饲植物雌激素对泌乳期母马增强免疫机能,提高机体抗氧化能力起到了一定作用,且补饲0.7g大豆异黄酮时对机体抗氧化能力效果较为明显。

The experiment was conducted to study the effects of phytooestrogens supplementation on blood biochemical indexes and antioxidant capacity of Yili maria during lactation.A total of 24 Yili maria during lactation were divided into four groups with similar age,parity,lactation months,i.e.groupsⅠ,Ⅱ,Ⅲ and control group.The control group and trial groups fed with the same based diet,groups Ⅰ,Ⅱ and Ⅲ were supplemented with 0.7g soybean isoflavone,0.7g genistein,0.35 g soybean isoflavone and 0.35 g genistein respectively.The total duration of the experiment was 90 d,the first 10 dof the experiment was the preliminary period and the formal experiment lasted for 80 d.The blood samples were collected by jugular vein in the experiment of0,20,40,60,80 days,respectively,3 500r/min centrifuged 15 min separate plasma and-20 ℃saved for later use.The results showed that compared with control group,total protein,albumin content and GSH-Px,SOD,CAT activity of blood in trail groups were no significant difference（P〉0.05）;But CAT,T-AOC activity of blood in trail groups had a upward trend.GLB,BUN content of blood in group Ⅱ were significantly higher than control group and group Ⅰ（P〈0.05）;no significant difference was observed among other groups（P〉0.05）.Compared with control group,the MDA content of blood in groupsⅠ and Ⅱ were trends to decrease,and groupⅠreached significant level（P〈0.05）.Therefore,phytooestrogens supplementation had enhanced immune function,improved the body＇s antioxidant capacity of Yili maria during lactation,when supplementary feeding 0.7g soybean isoflavone on antioxidant capacity of Yili maria during lactation was more obvious effect.