摘要
为了初步研究前期试验筛选获得的枯草芽孢杆菌产细菌素的生物学活性,本试验采用杯碟扩散法绘制出硫酸黏杆菌素和杆菌肽锌两种抗生素的抑菌效价标准曲线,用抗生素效价当量来评价细菌素的活性,并在不同温度处理下测定其抗菌活性和热稳定性。结果表明,125mg/mL细菌素溶液抑菌效价与26mg/mL硫酸黏杆菌素或11.9mg/mL杆菌肽锌溶液相当。此外,该细菌素在高温条件下(60~80℃)处理15min仍能维持较高抑菌活性。综上所述,本试验在细菌素和抗生素的抑菌活力之间建立了更直观的联系,并证明了该细菌素具有较好的热稳定特性,为其产业化推广提供了理论支持和技术借鉴。
The standard curve of two antibiotics(colistin sulfate and bacitracin zinc)was carried out to assess antimicrobial activity of bacteriocin,which obtained from our earlier work,using cup-dish method.In addition,we also observed the effects of the reaction temperature on the antibacterial activity of the bacteriocin.Results showed that 125mg/mL bacteriocin displayed similar antibacterial activity to 26mg/mL colistin sulfate or 11.9mg/mL bacitracin zinc.Furthermore,the bacteriocin remained good thermal stability under high temperature(nearly 60 to 80℃)in 15 minutes.Overall,this research built a direct relationship between this bacteriocin and antibiotics,and testified good stablility of this bacteriocin,providing theoretical support and technology reference to promote its large scale production.
出处
《中国畜牧兽医》
CAS
北大核心
2015年第12期3268-3273,共6页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金(31272476)
关键词
细菌素
抗生素
抑菌活力
热稳定性
bacteriocin
antibiotics
antibacterial activity
thermal stability