miR-141抑制头颈部鳞癌细胞增殖

miR-141 inhibits the proliferation of head and neck squamous cell carcinoma

目的考察microRNA-141(miR-141)对人头颈部鳞癌细胞增殖的影响。方法通过qRT-PCR分析19例头颈部鳞状细胞癌患者的肿瘤组织与癌旁正常组织标本中miR-141的表达。通过miR-141mimics或miR-141反义寡核苷酸(ASO)上调或抑制人喉癌细胞株(Hep-2)和人舌鳞状细胞癌细胞株(SCC-9)细胞中miR-141的表达水平。采用MTT实验分析上调或抑制miR-141的表达水平对Hep-2和SCC-9细胞增殖的影响。利用生物信息学方法预测miR-141的靶基因。结果 qRT-PCR的结果表明,头颈部鳞状细胞癌组织的miR-141的表达水平低于癌旁正常组织(P<0.05);上调miR-141的表达能够抑制Hep-2和SCC-9细胞的增殖(P<0.05),而抑制miR-141表达可以促进Hep-2和SCC-9细胞的增殖(P<0.05)。生物信息学方法预测miR-141的靶基因可能为ZEB1基因。结论 miR-141可能通过ZEB1基因抑制头颈部鳞状细胞癌的增殖。

Objective To study the effect of miR-141 on the proliferation of head and neck squamous cell carcinoma（HNSCC）cells.Methods The expressions of miR-141 in 19 HNSCC tissues and the matched adjacent normal tissues were analyzed by qRT-PCR.The miR-141 in Hep-2or SCC-9cells was overexpressed or down-regulated by miR-141 mimics or miR-141 ASO transfection.Then the effects of overexpression or down-regulation of miR-141 on cellular proliferation were analyzed by MTT experiment.The targeted genes of miR-141 were predicted by bioinformatics algorithms.Results The expression of miR-141 in HNSCC tissues was found significantly lower than that in matched normal tissue（P〈0.05）.Overexpression of miR-141 significantly inhibited the proliferation of Hep-2 and SCC-9cells,and down-regulation of miR-141 significantly promoted the proliferation of Hep-2and SCC-9cells（P〈0.05）.Bioinformatics algorithms showed that ZEB1 was likely to be a targeted gene of miR-141 in HNSCC.Conclusion miR-141 may inhibit HNSCC cell proliferation via ZEB1.