生慧汤对糖尿病脑病模型大鼠认知障碍及神经病理改变的影响

Effects of Shenghui Decoction on cognitive function and nerve pathological change in rats with diabetic encephalopathy

目的观察生慧汤(熟地黄、山茱萸、远志、酸枣仁、柏子仁等)对糖尿病脑病模型大鼠认知功能及神经病理改变的影响。方法 90只雄性SD大鼠,除空白组(n=15)外均给予高脂高糖饲料50 d,腹腔注射链脲佐菌素建立2型糖尿病脑病大鼠模型,随机均分为模型组、石杉碱甲组、吡拉西坦组、生慧汤低、高剂量组,给药30 d,Morris水迷宫法进行行为学测试和取血检测血糖,部分大鼠处死取脑制备海马组织匀浆,检测乙酰胆碱酯酶(Ach E)、糖基化终末产物(AGEs)和乙酰胆碱转移酶(Ch AT),其余大鼠取脑组织病理切片,并进行糖基化终末产物受体(RAGE)和核转录因子-κB(NF-κB)免疫组化染色。结果生慧汤可明显缩短模型大鼠的逃避潜伏期和探索距离;显著延长目标象限游泳时间,增多站台穿越次数;降低空腹血糖,增加大鼠体质量;升高海马组织中Ch AT活性,降低Ach E活性及AGEs水平;免疫组化染色显示生慧汤组海马组织齿状回区RAGE和NF-κB表达减少。结论生慧汤可明显改善糖尿病脑病模型大鼠认知障碍,胆碱能神经功能,海马组织病变,具有一定脑保护作用。

AIM To observe Shenghui Decoction（ Rehmanniae Radix praeparata,Corni Fructus,Polygalae Radix,Ziziphi spinosae Semen,Platycladi Semen,etc.） on cognitive function and nerve pathological change in rats with diabetic encephalopathy. METHODS Ninety male SD rats,except the blank group（ n = 15）,were given high fat and sugar feed,and intraperitoneal injection of STZ to establish type 2 diabetes encephalopathy rats.The modeled rats were equally and randomly divided into the model group,huperzine A group,piracetam group,Shenghui Decoction low and high dose group. All drugs were administrated for 30 days. The behavior tests were performed on the Morris water maze method. The rats blood glucose,part of rats brain hippocampus tissue homogenates were taken to measure the levels of Ch AT,Ach E and AGEs. The rest of rats＇ brain tissue were carried out on pathological observation（ n = 15） RAGE,and NF-kappa B immunohistochemical staining. RESULTS Shenghui Decoction obviously shortened the escape latency of model rats and explore distance,significantly extended target quadrant swimming time,increased the platform through the number,reduced fasting blood glucose,increased the weight of body,elevated Ch AT activity in the hippocampus,lessened Ach E and AGEs level. Immunohistochemical staining showed that Shenghui Decoction decreased hippocampus DG RAGE and the NF-kappa B expression. CONCLUSION Shenghui Decoction can obviously improve cognitive impairment,the function of cholinergic nerve,and hippocampal tissue lesions,with certain brain protection.