大蒜素与盐酸小檗碱联用体外抗菌试验

In vitro antibacterial activity of allicin in combination with berberine hydrochloride

目的研究大蒜素联合盐酸小檗碱对临床常见菌及耐药菌的抗菌效果。方法采用微量肉汤稀释法测定供试药物对4个常见菌,2个标准菌,和2个耐药菌的最小抑菌浓度（MIC）,微量棋盘稀释法和时间—杀菌曲线法评价两药对各试验菌株的联合作用。结果当两药单独应用时,其MIC值范围分别为大蒜素156.25（敏感株）-1250（耐药株）μg/m L,盐酸小檗碱78.125（敏感株）-312.5（耐药株）μg/m L。当两药联合应用时,部分抑菌浓度指数（FICI）值为0.75-1,为相加作用。从24 h时间—杀菌曲线可以发现,当两药单独应用时,即使在4×MIC下,也未能显示杀菌效应,而联用时在相对较低质量浓度下显示出杀菌作用;还显示出在低于MIC下具有弱协同（敏感株）或者相加作用（耐药株）。结论大蒜素和盐酸小檗碱联合应用,具有良好的抗菌作用。

AIM To study the in vitro activity of allicin（ dially trisulfide） in combination with berberine hydrochloride to common and drug-resistant bacteria. METHODS The broth dilution method was used to test individual minimal inhibitory concentrations（ MIC） of four common,two control and two resistent bacteria. The checkerboard dilution and time-kill curve tests were performed on antibacterial synergy effects. RESULTS The MIC of allicin were in the ranges of 156. 25（ sensitive bacteria） and ~ 1250 μg / m L（ drug-resistant bacteria）;the MIC of berberine hydrochloride were in the ranges of 78. 125（ sensitive bacteria） and ~ 312. 5 μg / m L（ drugresistant bacteria）. The fractional inhibitory concentration index（ FICI） of allicin plus berberine hydrochloride ranged from 0. 75 to 1. 24 h time-kill curve assay showed no bactericidal activity against all test strains even with4 × MIC used alone. However,when used together under the same concentrations,they showed bactericidal activity with synergy and additional effects. CONCLUSIONS The experimental data reveal that the combination of allicin and berberine hydrochloride have moderate antibacterial effect.