鄂西红豆离体培养及植株再生研究

Tissue culturing and plantlet regeneration of Ormosia hosiei Hemsl & Wils

【目的】对外植体采用特殊处理方法,并对不同培养时期的基本培养基进行调整,以建立高效、快速、繁殖系数高的鄂西红豆再生技术体系。【方法】以成熟度为80%的鄂西红豆种子为外植体,通过向培养基中添加不同质量浓度和配比的植物生长调节物质,筛选适宜的芽诱导培养基、继代增殖培养基、壮苗培养基和生根培养基,并对诱导生根培养的试管苗进行炼苗移栽,观察其成活率。【结果】最适芽诱导培养基为MS＋6-BA 0.5 mg/L＋KT0.5mg/L＋NAA 0.5mg/L＋维生素B10.1mg/L＋蔗糖30g/L＋琼脂6.5g/L＋活性炭1.5g/L,诱导率达85%;最适芽继代增殖培养基为WPM＋6-BA 1.5 mg/L＋KT 0.3 mg/L＋TDZ 0.02 mg/L＋NAA 0.5 mg/L＋蔗糖30g/L＋琼脂6.5g/L＋活性炭1.5g/L;无根苗壮苗培养基为WPM＋NAA 0.5 mg/L＋IBA 1.0 mg/L＋IAA 1.0mg/L＋蔗糖30g/L＋琼脂6.5g/L＋活性炭1.5g/L。当无根苗生长到4-5cm时形成完整植株并转接到生根培养基上诱导生根,最适生根培养基为1/2WPM＋IBA 0.5mg/L＋NAA 1.5mg/L＋新型基因诱导生根剂0.05mg/L＋蔗糖30g/L＋琼脂6.5g/L＋活性炭1.5g/L。【结论】建立的鄂西红豆再生技术体系可获得75%-85%的正常萌芽,生根率达85%以上,瓶苗移栽成活率达90%。

【Objective】An efficient,fast and high propagation coefficient sterile culturing system for Ormosia hosiei Hemsl Wils was established by treating explant with special methods and adjusting basic culture medium at different culturing periods.【Method】Using O.hosiei Hemsl Wils seeds with 80%maturity as explants,plant growth regulating substances with different concentrations and combinations were added in the medium to screen appropriate medium for bud induction,subculture multiplication,strong plantlet and rooting.The plantlets were also hardened and transplanted to get the survival rate.【Result】The optimum medium for bud induction was MS＋6-BA 0.5 mg/L＋KT 0.5 mg/L＋NAA 0.5mg/L＋B1Vitamin 0.1mg/L＋Sugar 30g/L＋Agar 6.5g/L＋Activated Carbon 1.5g/L and the obtained induction rate of embryo was 85%;the optimum medium for bud subculture multiplication was WPM＋6-BA 1.5mg/L＋KT 0.3mg/L＋TDZ 0.02mg/L＋NAA 0.5mg/L＋Sugar 30g/L＋Agar 6.5g/L＋Activated Carbon 1.5g/L;and the optimum medium for strong plantlet was WPM＋NAA 0.5mg/L＋IBA 1.0mg/L＋IAA 1.0mg/L＋Sugar 30g/L＋Agar 6.5g/L＋Activated Carbon 1.5g/L.A complete plant was formed when the bud grew up to 4-5cm and it was then transferred to the rooting medium,with the optimum medium of 1/2WPM＋IBA 0.5mg/L＋NAA 1.5mg/L＋ABT powder 0.05mg/L＋Sugar 30g/L＋Agar 6.5g/L＋Activated Carbon 1.5g/L.【Conclusion】The established sterile culturing system for O.hosiei Hemsl Wils obtained normal budding rate,rooting rate and plantlets transplanting survival rate of75%-85%,〉85% and 90%,respectively.