结直肠肿瘤组织中皮动蛋白的表达与临床病理意义及其与Arg和Tspan-1相关性的研究

Relative research on Leather protein expression plus clinical pathological significance and Arg plus Tspan-1 of colorectal tumor tissues

目的:研究皮动蛋白基因和TSPAN-1及Arg在结直肠癌组织中的表达,分析其与临床病理因素的关系。方法:采用免疫组化ELIVISION二步法,检测TSPAN-1和CORTACTIN基因及Arg蛋白在80例结直肠癌组织,13例结肠腺瘤组织及27例正常黏膜组织中的表达。结果:TSPAN-1蛋白在正常黏膜、结肠腺瘤、结肠癌中阳性表达率分别为7.4%、23.0%、90.0%,组间差异具有统计学意义(P<0.01);皮动蛋白在正常黏膜、结肠腺瘤、结肠癌中阳性表达率分别为52.0%、77.0%、97.5%,组间差异具有统计学意义(P<0.01),Arg蛋白在正常黏膜、结肠腺瘤、结肠癌中阳性表达率分别为11.1%、30.8%、91.2%,组间差异具有统计学意义(P<0.01);皮动蛋白和TSPAN-1及Arg的表达与结肠癌的淋巴结转移、TNM分期、五年生存率、组织学分级及浸润深度有关(P<0.01);皮动蛋白和TSPAN-1及Arg在结肠癌组织中的表达强度呈正相关(r=0.397,P=0.0013)(r=0.397,P=0.0013),三者表达的一致率高达88.8%(71/80)及90.0%(72/80)。结论:皮动蛋白基因的高表达与肿瘤细胞的侵袭性转移性有关,可以被用作判断结直肠癌预后的一个标志。皮动蛋白与TSPAN-1基因的高表达与结肠癌的侵袭和发展有关,二者在结肠癌的细胞增殖、浸润和转移中有协同作用,联合检测二者的表达对于进一步理解结肠癌的生物学行为和判断预后有一定价值。皮动蛋白与TSPAN-1可能作为结直肠癌检测的肿瘤标记物和治疗的分子靶点。Arg的检测对皮动蛋白与TSPAN-1基因的联合检测有协同辅助作用,三者的检测更具有准确性。

Objective： To research the expression of Arg and TSPAN-1 in colorectal carcinoma tissues, and analyze its relationship with the clinical pathological factors. Methods： The expression of TSPAN-1 and CORTACTIN gene and Arg protein in 80 cases of colorectal cancer tissues, 13 cases of colorectal adenoma tissues and 27 cases of normal mucosa tissues were detected by two step method of immunohistochemical ELIVISION. TSPAN-1 protein expression in the normal mucosa, colorectal adenomas, colon cancer, the positive expression rate was 23.0%, 90.0% and 30.8%, respectively, the positive expression rate of P was 52.0%, 77.0%, 97.5%, Arg protein expression in normal mucosa, colorectal adenoma, colorectal cancer, P〈0.01 protein expression and TSPAN-1, Arg and TSPAN-1, TNM, Arg, P〈0.01, and, P〈0.01 and. The expression level of P=0.0013（r=0.397）（r=0.397, P=0.0013）, and the expression of the three groups were 88.8%（71/80） and 90.0%（72/80）. Conclusion： The high expression of the gene is related to the invasion and metastasis of tumor cells, and it can be used as a marker for the prognosis of colorectal cancer. The high expression of gene and TSPAN-1 gene were related to the invasion and development of colon cancer. The two had a synergistic effect on cell proliferation, invasion and metastasis of colon cancer. TSPAN-1 may serve as a molecular target for tumor markers and therapeutic targets for colorectal cancer detection. The detection of Arg and TSPAN-1 gene in combination with the detection of synergistic effect, the detection of the three more accurate.