纤维素酶酶解法提取怀山药多糖工艺研究

Study on Extraction of Polysaccharide from Dioscorea Opposita Thunb by Enzymatic Hydrolysis of Cellulase

以怀山药为材料,采用纤维素酶酶解,提取其活性成分多糖,先通过单因素实验考察了料水比、酶添加量、酶解时间,酶解温度对怀山药多糖提取得率的影响;然后通过正交试验对怀山药多糖的酶解提取工艺进行了优化,结果表明各因素对怀山药多糖提取率影响的主次顺序依次为:酶添加量、酶解时间、酶解温度、料水比;其最优提取工艺条件为:酶添加量3%,酶解时间75 min,酶解温度60℃,料水比1∶30,在此最优工艺条件下,怀山药多糖的提取率为15.78%。

The Polysaccharide was extracted from Dioscorea opposita Thunb by enzymatic hydrolysis of Cellulase. First, the effects of the ratio of material and water, the amount of the enzyme, the time of enzyme hydrolysis, and the temperature of enzyme hydrolysis on the extraction rate of Polysaccharide from Dioscorea opposita Thunb were studied by single factor experiment. Then the extraction process of Polysac-charide from Dioscorea opposita Thunb was optimized by orthogonal test. The results showed that the order of the primary and secondary factors affecting the extraction rate of Polysaccharides from Dioscorea opposi- ta Thunb was in order：the amount of the enzyme, the time of enzyme hydrolysis, the temperature of enzyme hydrolysis,the ratio of material and water. The optimal extraction conditions were as follows ： the amount of the enzyme 3%, the time of enzyme hydrolysis 75 min,the temperature of enzyme hydrolysis 60 ℃ ,the ratio of material and water 1： 30. The Polysaccharide yield of 15.78% was achieved under these conditions.