母代高脂饮食对F2代大鼠的不良代谢影响

Adverse effect of maternal high fat diet on metabolism in the F2 generation rats

目的观察母代高脂饮食对F2代大鼠的不良代谢影响。方法 3周龄SD雌性大鼠随机分为高脂饮食组(F0-HF组)和对照组(F0-CON组),至11周龄时交配怀孕产仔(F1代),母鼠按原饲养要求喂养至哺乳期结束。F1代大鼠断乳后均予普通饲料喂养至11周龄时再次交配产仔(F2-HCC组和F2-CON组),两组F2代大鼠均普通饲料喂养至3周龄,观察其糖脂代谢相关指标变化。结果 F2-HCC组大鼠出生、1周龄时体质量明显高于对照组大鼠,差异有统计学意义(P均<0.05);F2-HCC组大鼠3周龄时空腹血糖和糖耐量曲线下面积均高于对照组,血清总胆固醇和肝脏三酰甘油水平均高于对照组,差异有统计学意义(P均<0.05);1日龄及3周龄时F2-HCC组大鼠表现出不同程度的脂肪肝,对照组大鼠肝脏镜下结构均正常。结论母代高脂饮食可引起F2代大鼠体质量增加、肝脏脂肪变性及糖耐量受损,后代发生代谢性疾病的危险性增加。

Objective To examine the effects of maternal high-fat（HF） diet on the development of metabolic diseases in their offspring. Methods Female Sprague-Dawley rats were fed either a high fat diet（F0-HF） or control（F0-CON） diet for 8 weeks before mating and throughout gestation and first 3 weeks of lactation. At 11 weeks of age, F1 females were mated to produce the F2 generation（F2-HCC and F2-CON）. The metabolic markers and the liver pathology were examined in the F2 generations at 3 weeks of age. Results The F2-HCC had a significantly higher birth weight and body weight at one week of age than F2-CON（P〈0.05,respectively）. At 3 weeks of age, the fasting blood glucose level and glucose tolerance area under the curve（AUC） in F2-HCC were significantly higher than those in F2-CON（P〈0.05,respectively）; the levels of total serum cholesterol and liver triglycerides were significantly higher in F2-HCC than those in group F2-CON（P〈0.05,respectively）. At birth and 3 weeks of age, the histological examination showed different degrees of fatty liver in F2-HCC, but normal structure in F2-CON. Conclusions Maternal HF diet may result in increased body weight, fatty liver and impaired glucose tolerance in the F2 offspring.