血清神经肽Y和骨桥蛋白在肝细胞肝癌诊断中的应用研究

Diagnostic application and comparative analysis of serum Neuropeptide Y and Osteopontin in hepatocellular carcinoma

目的:探讨神经肽Y(Neuropeptide Y,NPY)和骨桥蛋白(Osteopontin,OPN)在肝细胞肝癌(hepatocellular carcinoma,HCC)诊断中潜在的应用价值。方法:收集各类受试者血清,其中慢性乙型肝炎(chronic hepatitis B,CHB)65例、肝硬化(liver cirrhosis,LC)74例、HCC患者53例、健康志愿者68例作为正常对照(health control,HC)。以酶联免疫法检测受试者血清中NPY、OPN和α-甲胎蛋白(alpha-fetoprotein,AFP)含量,分析NPY、OPN在HCC诊断中的应用价值并与AFP进行比较。结果:HCC患者血清NPY显著高于HC组、CHB组及LC组(F=59.63,P<0.0001);HCC患者血清OPN显著高于HC组、CHB组及LC组(F=36.00,P<0.0001);与HC组相比,受试者工作曲线(ROC)下面积分别为NPY=0.924±0.025,OPN=0.864±0.036;NPY对HCC诊断灵敏度和特异度分别为79.28%、98.53%;OPN对HCC诊断灵敏度和特异度分别为83.02%、82.35%,与AFP相比,NPY特异性最强,OPN灵敏度最高。结论:血清NPY和OPN是潜在的HCC诊断标志物,与AFP联合诊断对HCC的诊断价值更高。

Objective：To explore the diagnostic value of Neuropeptide Y（NPY） and Osteopontin（OPN） in patients with hepatocellular carcinoma（HCC）. Methods：Serum of 65 patients with chronic hepatitis B（CHB）, 74 patients with liver cirrhosis（LC）, 53 patients with HCC, and 68 health people as control（HC） were collected. Serum levels of NPY, OPN, and alpha-fetoprotein（AFP） were detected by enzyme linked immunosorbent assay（ELISA）. The value of them in the diagnosis of HCC was analyzed by receiver operating characteristic curve（ROC）. Results： Serum NPY（F=59.63, P〈0.0001） and OPN（F=36.00, P〈0.0001） levels were significantly higher in HCC patients than in HC, CHB and LC. Area under ROC curve of NPY is 0.924±0.025 and OPN is 0.864±0.036. The specificity and sensitivity of NPY are 79.28% and 98.53%, OPN are83.02% 、82.35%. Compared with AFP, the specificity of NPY is the strongest and the sensitivity of OPN is the highest.Conclusion： Serum NPY and OPN is a potential diagnostic marker for HCC. The combined use of NPY, OPN, and AFP as biomarker panel could enhance the diagnostic ability for HCC.