P63和Ki67在宫颈上皮内瘤变和宫颈癌中的表达及意义

Expression and clinical significance of P63 and Ki67 in patients with cervical cancer and cervical intraepithelial neoplasia

目的:探讨P63、Ki67在宫颈癌和宫颈上皮内瘤变中的表达及临床意义。方法:采用免疫组化PV-9000二步法,检测P63、Ki67在17例宫颈癌、34例宫颈上皮内瘤变和10例正常宫颈组织中的表达。结果:P63在CIN I、II、III及宫颈浸润癌阳性表达率分别为42.9%、77.8%、94.4%、100.0%,Ki67的表达率分别为57.1%、77.8%、100.0%、100.0%。而P63在CIN各级及宫颈浸润癌中表达"++"以上比例分别为14.3%、44.4%、61.1%、76.5%,Ki67分别为28.6%、33.3%、66.7%、88.2%。P63、Ki67在正常宫颈组织、宫颈上皮内瘤变、宫颈癌中阳性表达率随病情的加重而逐渐升高(P<0.05)。结论:P63和Ki67联合检测可作为早期诊断CIN、宫颈癌的标志物,可提高宫颈癌的早期诊断率。

Objective：To investigate the expression and their clinical significance of P63 and Ki67 in the tissues of cervical cancer and cervical intraepithelial neoplasia（CIN）. Methods：Protein expressions of P63 and Ki67 in 17 patients with cervical cancer, 34 CINs and 10 normal cervical tissues were detected, using PV-9000 two-steps immunohistochemical assay. Correlations of the expression among them were analyzed. Results： The rates of P63 expression in patients with CIN I, CIN II, CIN III, and invasive cervical cancer（ICC） were 42.9%, 77.8%, 94.4%, and 100%, respectively. The positive expression of Ki67 in those patients were 57.1%, 77.8%, 100%, and 100%, respectively. The expression magnitude of P63 protein in 14.3%CIN I, 44.4%CIN II, 61.1%CIN III, and 76.5% ICC patients exceeded ＂＋＋＂,while Ki67 protein expression were more than ＂＋＋＂in 28.6%CIN I, 33.3%CIN II, 66.7%CIN III, and 88.2%ICC patients.The positive expression rates of P63、Ki67 were rose gradually with their degree of severity significantly（P〈0.05）. Conclusion：These findings have shown that the expression of P63 and Ki67 by the Joint testing could be a reliable clinical marker for early disgnosis of CIN and would play an important role in estimating the biological action and prognosis of cervical cancer.