一株拮抗稻瘟病内生链霉菌OsiSh-10的筛选与鉴定

Screening and Indentification of an Endophytic Streptomyces to Antagonize Rice Blast

从湖南浏阳大围山种植的湘矮早7号水稻中,筛选到一株对水稻稻瘟病菌有显著抑制效果的水稻内生放线菌OsiSh-10菌株.由拮抗菌包覆种子和喷晒水稻叶面的实验表明将该菌喷晒于水稻叶面可以对叶瘟起到明显的控制效果,且喷一次的效果比喷两次的效果好,包覆种子反而使病情指数升高.根据形态特征、培养特征、生理生化、16s rRNA序列比对及进化树构建鉴定OsiSh-10菌株属于白色链霉菌(Streptomyces albus).OsiSh-10是一株具有稻瘟病生防潜力的菌株.

An endophytic actinomycete strain OsiSh-10 significantly against rice blast pathogens was successfully isolated from Xiangaizao 7 rice （Oryza indica） of Liuyang Dawei Mountain in Hunan Province. The control effects of OsiSh-10 on leaf blast in field were investigated by coating seeds and spraying leaf of rice with it, which were as follows, leaf blast was controlled by spraying OsiSh-10 on leaf of rice , but coated seeds with OsiSh-10 unworked for leaf blast. According to its morphological features, culture characteristics, physiological and biochemical characteristics, 16s rRNA gene sequence comparison, and phylogenetic tree construction analysis, OsiSh-10 strain was promising biocontrol actinomycete for rice blast. classified as Streptomyces albus. OsiSh-10 strain is a promising biocontrol actinomycete for rice blast.