基于基因表达谱芯片杂交分析免疫前后七鳃鳗白细胞的基因表达差异

Microarray analysis of gene expression profiling in leukocyte associated with immune response in Lamprey

七鳃鳗作为现存的圆口纲无颌类脊椎动物的代表,已成为研究脊椎动物适应性免疫系统起源和进化的新型模式生物.为筛选七鳃鳗白细胞中免疫防卫相关基因,利用人基因芯片分析脂多糖(LPS)免疫七鳃鳗前后白细胞中基因的差异表达.研究结果表明:免疫后七鳃鳗白细胞中共有155条差异表达基因,其中,上调的基因共92条,下调的基因共63条,涉及细胞生长分化、信号转导、细胞代谢、免疫防卫、细胞凋亡、增殖等多个方面.本研究首次应用人基因表达谱芯片杂交分析免疫前后七鳃鳗白细胞的基因表达差异,为探索无颌类免疫应答机制提供新的有价值的线索.

Lampreys,the surviving representative of jawless vertebrates,have been a focal point in the research for the evolutionary origin of adaptive immunity.In this study,to determine the cellular gene expression changes in lamprey leukocytes in response to lipopolysaccharide（LPS）treatment,Affymetrix GeneChip？Human Genome U133 Plus 2.0 Array were used to screen transcriptome differences.Expression levels of 155 genes were significantly altered by at least two-fold upon treatment with LPS compared with controls.92 of the 155 genes were up-regulated and 63 genes were down-regulated,which involved in cell growth and differentiation,signal transduction,cell metabolism,immune defense,apoptosis,proliferation,and many other aspects.The results obtained in this study provide the first example of using a human whole-genome platform to correlate gene expression changes in lamprey leukocytes after the animal was stimulated with LPS,which is helpful to shed more light in the immune response of the oldest jawless vertebrates.