摘要
在GenBank中获得非洲爪蟾Caveolin-1蛋白序列,通过多种生物信息学分析软件对其进行分析,预测其结构和功能.以成年中华大蟾蜍为实验材料,提取蟾蜍不同肌肉组织的总蛋白,通过Western Blot技术检测Caveolin-1在肌肉组织中的表达.结果表明:蟾蜍的Caveolin-1与哺乳动物Caveolin-1有着相似的结构和功能;利用本实验室已有的抗人Caveolin-1多克隆抗体在中华大蟾蜍的不同肌肉组织中检测到了Caveolin-1的表达,从另一个角度验证了Caveolin-1的保守性.
The Caveolin-1protein sequence of Xenopus laevis in GenBank was obtained and its structure and function were predicted through various bioinformatics analysis softwares.The results showed that Caveolin-1of toad and mammals have similar structures and functions.Total protein from muscle tissues of Bufo gargarizans was extracted and the Caveolin-1had been detected through Western Blot by mouse anti-human Caveolin-1polyclonal antibody.It was suggested that Caveolin-1is highly conserved in the long biological evolution process,and maintains its function as a signal transduction center.
出处
《辽宁师范大学学报（自然科学版）》
CAS
2015年第4期526-530,共5页
Journal of Liaoning Normal University:Natural Science Edition
基金
国家自然科学基金项目(30970353)
