抗痛蝎毒素多肽的重组表达研究

Recombination of Anti- pain Scorpion Toxin Polypeptides

蝎毒素多肽作用于众多细胞膜离子通道,调控通道动力学特性。显具抗痛活性的蝎毒素多肽呈现较高的学术研究价值和医药应用前景。序列比对分析发现,抗痛蝎毒素多肽具较高保守型,序列同源性达45%,均具8个半胱氨酸残基,且其位置相对保守,这可能与抗痛功能有关。天然蝎毒素蛋白的获得、结构与功能研究受限,而高纯度、高活性重组毒素多肽是一条有效解决途径。本文综述抗痛蝎毒素多肽的重组表达,藉此推进其结构与功能相关性的理解。

Scorpion toxin peptides target on and regulate gating dynamics of multiple ion channels. Anti- pain scorpion toxin polypeptides are a kind of potential analgesic drugs. Sequence alignment analysis showed that the anti-scorpion toxin polypeptide performed about 45% sequence homology and three dimensional topology all combined by eight conservative scaffold cysteine residues,which may be corresponding to their anti- pain function. Moreover,gene recombinant and mutation of high- purity and high- activity polypeptides would be the more effective pathways to elucidate the conservation and active properties. The paper thus reviews the current recombination progressing and application of scorpion polypeptides to further our understanding to the structure- function relationship of natural anti- pain scorpion toxins.