正交试验法对樟树根中总黄酮提取工艺的优选

Optimization of Technology of Extracting Total Favonoids in Camphor Tree Root Orthogonal Test

以樟树[Cinnamomum camphora(L.)Presl.]根为原料,利用超声波辅助提取樟树根中的总黄酮成分。在单因素试验的基础上,采用正交试验法,重点考察了超声波功率、提取温度、提取时间和料液比等因素对总黄酮提取率的影响,并最终确定最佳工艺条件为:超声功率为480 W,提取温度为50℃,料液比为1∶25(g∶m L),提取时间为30 min,采用该最佳提取工艺,总黄酮的提取率可达13.50%。

The extraction of flavonoids from Camphor tree Root with ultrasonic wave was studied. On the basis of mono factor and orthogonal experimental design, the influence of several parameters on the extraction rate of total flavonoids were investigated importantly, including ultrasonic power, ultrasonic extracted temperature, ultrasonic extracted time, and material/extraction solution ratio etc. And the optimum extraction conditions were as follows： ultrasonic power 480 W,ultrasonic extracted temperature 50 ℃,material/extraction solution ratio 1：30 and ultrasonic extracted time 30 min. Under the above optimum conditions,The total flavonoids yielded 13.50%.