腹腔内注射脂多糖对大鼠黑质ROS含量及NADPH酶的影响

Effect of intraperitoneal injection of lipopolysaccharides on the substantia nigra NADPH oxidase in rats

目的观察腹腔注射脂多糖(lipopolysaccharide,LPS)后,大鼠黑质还原型烟酰胺腺嘌呤二核苷酸磷酸(nicotinamideadenine dinucleotide phosphate,NADPH)氧化酶的表达及活性氧自由基(reactive oxygen species,ROS)的产生情况。方法健康2月龄、1年龄SD大鼠经腹腔注射LPS(1 mg/kg)后,分别于6 h、12 h、24 h、48 h、1周处死,快速取脑黑质,用DCFH-DA探针检测各组大鼠ROS产生,用Western blotting法检测NADPH酶在胞质蛋白和膜蛋白的表达情况。结果腹腔注射LPS 12 h,大鼠黑质内ROS活性达到高峰,NADPH酶活性增加,达到高峰。结论无论在老年组与青年组,LPS刺激后(12 h)的氧化应激反应均可能是通过激活NADPH氧化酶途径而发生的。

Objective To observe the expression of nicotinamide-adenine dinucleotide phosphate（ NADPH） oxidase and the production of reactive oxygen species（ ROS） in substantia nigra after intraperitoneal injection of lipopolysaccharide（ LPS） in rats.Methods Healthy two-month-old and one-year-old SD rats were intraperitoneally injected with LPS（ 1 mg / kg） at 6 h,12 h,24 h,48 h,and 1 week,respectively. Substantia nigra was taken,reactive oxygen species（ ROS） and the activity of NADPH oxidase in the rats were determined. Results At 12 h after intraperitoneal injection with LPS,the activity of ROS in substantia nigra reached the peak and NADPH activity also reached the peak. Conclusion The increase in expression of NADPH oxidase and the shift to membranes protein promotes the inflammatory reaction,however there was no significant differences between the aged group and the young group.