高效毛细管电泳分离美托洛尔对映体

Separation and analysis of metoprolol enantiomers by high performance capillary electrophoresis

建立HPCE手性分离琥珀酸美托洛尔和酒石酸美托洛尔对映体的方法。采用HPCE-CZE模式,在220 nm波长下,以β-CD为手性试剂,成功分离了β1受体阻滞剂琥珀酸美托洛尔和酒石酸美托洛尔对映体。考察了影响样品手性分离的主要因素:缓冲液种类、p H、浓度、分离电压、手性试剂的选择等,最终得到可以同时分离两种美托洛尔盐的电泳条件:用125 mmol/L,p H9.3的硼砂-Na OH溶液(含10 mmol/Lβ-CD,体积分数2%甲醇)作为电泳运行液,分离电压20k V。使用上述方法使得琥珀酸美托洛尔和酒石酸美托洛尔的对映体都达到基线分离,分离度分别是2.14和2.23。该方法适用于琥珀酸美托洛尔和酒石酸美托洛尔对映体的分离,亦可作为定量分析的依据。

A HPCE method was developed for the chiral separation and analysis of metoprolol enantiomers. The β1-blockers metoprolol succinate and metoprolol tartrate were separated successfully by the capillary zone electrophoresis （CZE） with PDA detection at 220 nm and β-CD as chiral reagent. Effects of the main factors affecting the HPCE ehiral separation, including composition of the running buffer, pH value, concentration of the buffer, separation voltage, and type of the ehiral reagent etc, were investigated. The baseline separation of the metoprolol enantiomers was obtained with 125 mmol/L borax-NaOH （ pH9.3 ） ＋ 2% methanol ＋ 10 mmol/L β-CD as the running buffer and the separation voltage is 20kV. The method mentioned above was used to separate the β-blockers metoprolol succinate and metoprolol tartrate successfully, and it could also be used for quantitative analysis of the metoprolol enantiomers.