Th1-Th2-Th17细胞因子在小鼠慢性心肌炎中的表达

Expressions of Th1-Th2-Th17 cytokines in mice chronic viral myocarditis

目的:观察Th1-Th2-Th17细胞因子在小鼠慢性心肌炎(VMC)模型中的表达,探究其在慢性VMC中的作用及其意义。方法:用柯萨奇病毒B3(CVB3)建立BALB/c VMC小鼠模型,实验组(n=15)腹腔注射磷酸缓冲液(Phosphate-buffered saline,PBS)0.1 ml,含102TCID50 CVB3病毒液;对照组(n=10)腹腔注射PBS 0.1 ml,不含病毒液。在注射后的第8周,心肌HE染色观察其病理形态特征;Masson's染色法观察心肌组织纤维化程度;检测血浆中Th1-Th2-Th17细胞因子含量及心肌组织中上述细胞因子的mRNA表达情况。结果:病毒感染8周后心肌病理符合慢性VMC病理特征;与对照组比较,血浆及心肌中IL-2、IL-5、IL-10、IL-13、IL-17、IL-6、IL-22、IL-21及TGF-β均明显升高(P均<0.05)。结论:Th1-Th2-Th17细胞因子失衡导致的免疫紊乱可能在慢性VMC发病机制中起重要作用。

Objective： To investigate the expressions of Th1-Th2-Th17 cytokines in the coxsackievirus B3-induced mice chronic viral myocarditis（ VMC）. Methods： BALB / c mice were intraperitoneally（ i. p） infected with increased CVB3 for establishing chronic VMC models. Control mice were treated with phosphate-buffered saline（ PBS） i. p. Cardiac tissues were obtained 8 weeks after CVB3 injection,myocardial histopathologic changes were observed by HE and Masson staining. Th1-Th2-Th17 cytokines in plasma were detected by protein array technology,and their cardiac mRNA expressions were measured by RT-PCR. Results： Compared with the control group,levels of IL-2,IL-5,IL-10,IL-13,IL-17,IL-6,IL-22,IL-21 and TGF-β obviously increased in chronic VMC group（ P all 0. 05）. Conclusion： The imbalance of Th1-Th2-Th17 cytokines may play an important roles in the pathogenesis of chronic VMC.