SATB2、NANOG和OCT4在口腔鳞癌中的表达及临床意义

Expression and clinical significance of SATB2,NANOG and OCT4 in oral squamous cell carcinomas

目的探讨特异AT序列蛋白2(special AT-rich sequence binding protein 2,SATB2)、胚胎干细胞关键蛋白(NANOG)、八聚体结合转录因子(octamer-binding transcription factor,OCT4)在口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)中的表达及其与口腔鳞状细胞癌临床病理特征的关系。方法采用免疫组织化学染色法检测SATB2、NANOG和OCT4在口腔鳞状细胞癌及正常黏膜中的表达,结合临床资料,对其与口腔鳞状细胞癌临床病理特征的关系进行统计分析。结果 SATB2、NANOG、OCT4在口腔癌组的表达明显高于正常组,且差异均有统计学意义(P<0.05)。口腔鳞癌组织中SATB2、NANOG和OCT4的表达与组织分化程度、TNM分期以及淋巴结转移之间关系无统计学意义(P>0.05),SATB2和OCT4表达与肿瘤的局部浸润无关(P>0.05),而NANOG表达则与肿瘤的局部浸润相关(P<0.05)。结论 SATB2、NANOG和OCT4在口腔鳞癌组织中高表达,可能与口腔鳞癌的发生发展有关。NANOG表达与肿瘤侵袭有关,可能在促进口腔癌侵袭转移中发挥重要作用。

Objective To investigate the expression of specific sequence AT protein 2（ SATB2）,NANOG,octamer-binding transcription factor（ OCT4） in oral squamous cell carcinoma（ OSCC） and its relationship with clinical pathological characteristics of oral squamous cell carcinoma. Methods Immunohistochemistry was used to examine the expression of SATB2,NANOG and OCT4 in oral squamous cell carcinoma and normal oral mucosa. Results The expressions of SATB2,NANOG and OCT4 were higher in OSCC than in normal oral mucosa,and the difference was statistically significant（ P〉0. 05）. The expression of SATB2,NANOG and OCT4 protein was unrelated with tumor grade,clinical stage,neck lymph-node metastasis（ P〉0. 05）,and the expression of SATB2,OCT4 protein was unrelated with local invasion（ P〉0. 05）. The expression of NANOG protein was related with local invasion（ P〈0. 05）. Conclusions SATB2,NANOG and OCT4 were highly expressed in oral squamous carcinoma tissues,which could be a tumor marker and used as targets of treatment. The expression of NANOG was associated with tumor invasion ability; it may play an important role in promoting the invasion and metastasis of OSCC.