生物活性玻璃与铒激光联用对磨切牙本质模型的封闭效果

Occluding effect of bioactive glass combined with Er laser irradiation on abrasion cutting dentine model

目的研究生物活性玻璃与铒激光联用对磨切牙本质模型的封闭效果。方法制取45个离体牙的牙本质盘,面牙本质按粗磨、细磨、抛光的顺序打磨平整,构建磨切牙本质模型,按处理方法不同随机分为5组:空白对照组(A),生物玻璃组(B),Er,Cr:YSGG激光组(C),生物玻璃+Er,Cr:YSGG激光组(D),Er,Cr:YSGG激光+生物玻璃组(E)。通过扫描电镜(SEM)和其附属X射线能谱分析(EDXA)比较5组封闭牙本质小管效果及封闭物组成。结果 SEM观察发现B、C、D、E 4组绝大部分封闭物堵塞牙本质小管,4组的封闭效果均较好。小管面积暴露率:D(0.044%±0.003%)<E(0.256%±0.012%)<C(0.628%±0.063%)<B(0.836%±0.015%)<A(5.694%±0.439%)。EDXA结果显示B、D、E组小管口封闭物含特殊元素Si。结论含有玷污层的磨切牙本质模型在生物玻璃和铒激光联用后,封闭效果达到最佳状态。

Objective To evaluate the occluding effect of bioactive glass combined with Er laser irradiation on abrasion cutting dentine model. Methods 45 dentine discs were prepared and the treating sides were roughly grinded,finely grinded and polished. They were randomly divided into 5 groups with different treatments： Group A（ no treatment as a control）,Group B（ bioactive glass）,Group C（ Er,Cr： YSGG laser irradiation）,Group D（ bioactive glass followed by Er,Cr： YSGG laser irradiation）,and Group E（ Er,Cr： YSGG laser irradiation followed by bioactive glass）. The occluding effect and mineral content were examined by scanning electron microscopy（ SEM） and X-ray energy spectrum analysis（ EDXA）. Results Micrographs of SEM showed excellent occluding effect in Group B,C,D and E,and the dentinal tubule exposure rates were D（ 0. 044% ± 0. 003%） 〈E（ 0. 256% ± 0. 012%） 〈C（ 0. 628% ± 0. 063%） 〈B（ 0. 836% ± 0. 015%） 〈A（ 5. 694% ± 0. 439%）. EDXA showed that special mineral content＂Si＂appeared in Group B,D and E. Conclusions The best occluding effect can be obtained when abrasion cutting dentine model with a smear layer was treated by bioactive glass combined with Er laser irradiation.