特异性细胞粘附Arg-Gly-Asp-Cys/三乙二醇单-11-巯基十一烷基醚界面的制备与电化学表征

Preparation and Electrochemical Characterization of Arg-Gly-Asp-Cys / Triethylene Glycol Mono-11-mercaptoundecyl Ether Interface for Specific Adhesion of Living Cells

采用自组装技术将人工合成的细胞粘附分子精氨酸-甘氨酸-天冬氨酸-半胱氨酸(RGDC)和防止细胞非特异性粘附的三乙二醇单-11-巯基十一烷基醚(TGME)修饰于金电极表面,制备了致密的、稳定的、对细胞具有特异性粘附作用的传感界面。通过调整RGDC与TGME的比例,比较了其对大鼠心肌细胞(H9C2)的特异性粘附效果。以铁氰化钾为探针的实验结果表明,该界面对大鼠心肌细胞有很好的特异粘附作用。此外,还考察了该界面在Cr(Ⅵ)溶液中的电化学行为。结果表明,随着Cr(Ⅵ)浓度的增高氧化还原峰的峰电流增大,而峰电位均负向移动。其电化学参数变化与显微镜下观察到的Cr(Ⅵ)对细胞形态的模拟结果表明,所构建的细胞特异性粘附界面及电化学分析技术可望用于重金属对细胞的毒性研究。

In this work,a compact and stable interface for specific adhesion of living cells was prepared through the self-assembly technology. The Arg-Gly-Asp-Cys（ RGDC） which can improve cell adhesion and triethylene glycol mono-11-mercaptoundecyl ether（ [OH-（ CH2-CH2-O）3-（ CH2）11-SH ], TGME） which prevents non-specific adhesion were decorated on the Au electrode. The adhesion efficiency of rat cardiac myocytes（ H9C2） onto the interface was studied by adjusting the ratio of RGDC and TGME. Moreover,the experiments by K3Fe（ CN）6served as a probe had demonstrated that the RGDC / TGME interface can effectively and specifically adheres the H9C2. In addition,the electrochemical behaviors of the interface in different concentrations of Cr（ VI） solutions were also investigated. Cyclic voltammograms showed that the redox peak current increased while the peak potential shifted negatively as the increase of Cr（ Ⅵ） concentration. And the electrochemical results were discussed with the morphology changes observed with an inverted electron microscopy,which indicates that the built living cells /electrochemical interface could potentially be used for the study of toxicity of heavy metals to living cells.