摘要
Magnetic lipase was prepared by a facial and cost-effective method. Lipase from Pseudomonase cepacia was covalently linked to Fe304 nanoparticles, which were produced by co-precipitating Fe^2＋ and Fe^3＋ ions in ammonia solution and subsequent coating with 3,4-dihydroxylaldehyde. Magnetic Fe304 nanoparticles modified with 3,4-dihydroxylaldehyde bonded lipase efficiently（47 mg/g）. The resulting magnetic-lipase is active（0.68 μmol·min^-1·mg^-1） and enantioselective（e.e, of the product 〉98%） in the hydrolysis of racemic 1-phenylethyl acetate. Furthermore, as a heterogeneous catalyst, the magnetic-lipase can be magnetically recycled, and a retained activity of 48% was obtained even after 6 cycles.
Magnetic lipase was prepared by a facial and cost-effective method. Lipase from Pseudomonase cepacia was covalently linked to Fe304 nanoparticles, which were produced by co-precipitating Fe^2＋ and Fe^3＋ ions in ammonia solution and subsequent coating with 3,4-dihydroxylaldehyde. Magnetic Fe304 nanoparticles modified with 3,4-dihydroxylaldehyde bonded lipase efficiently（47 mg/g）. The resulting magnetic-lipase is active（0.68 μmol·min^-1·mg^-1） and enantioselective（e.e, of the product 〉98%） in the hydrolysis of racemic 1-phenylethyl acetate. Furthermore, as a heterogeneous catalyst, the magnetic-lipase can be magnetically recycled, and a retained activity of 48% was obtained even after 6 cycles.
基金
Supported by the National Natural Science Foundation of China（No.21203017）, the Open Fund of State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences（No.N-11-3）, the Program for Liaoning Excellent Talents in University, China and the Fundamental Research Funds for the Central Universities, China（No.DC201502020304）.
