不同抗菌脂肽添加物对吉富罗非鱼增重率、血清生化指标和非特异性免疫力的影响

Effects of different types of antimicrobial lipopeptides supplementation on weight gain rate,serum biochemical indexes and non-specific immune ability of GIFT strain of nile tilapia(Oreochromis niloticus)

为了研究饲料中不同抗菌脂肽添加物对吉富罗非鱼增重率、血清生化指标和非特异性免疫力的影响,将240尾吉富罗非鱼(平均体重为2.01±0.03 g)随机分为4个组,即对照组(基础饲料)、抗菌脂肽组Ⅰ(基础饲料+50 mg/kg抗菌脂肽)、抗菌脂肽组Ⅱ(基础饲料+枯草芽孢杆菌发酵产物,抗菌脂肽水平为50mg/kg)和黄霉素组(基础饲料+10 mg/kg黄霉素),试验期为7周。结果发现,不同抗菌脂肽添加物均可显著提高吉富罗非鱼特定增长率、血清白蛋白水平、血清溶菌酶活性、免疫球蛋白M水平和补体C3水平,显著降低血尿素氮、总胆固醇、甘油三酯和低密度脂蛋白水平,对谷丙转氨酶活性、谷草转氨酶活性、高密度脂蛋白水平无显著影响;不同抗菌脂肽添加物组间的上述指标无显著差异。表明不同抗菌脂肽添加物对吉富罗非鱼的促生长作用及血清生化指标和非特异性免疫力的改善效果接近。

A 7-week feeding trial was conducted to evaluate the effects of different types of antimicrobial lipopeptides supplementation on the weight gain rate,serum biochemical indexes and non-specific immune ability of genetically improved farmed tilapia（GIFT,Oreochromis niloticus）. 240 fishes with initial average weight of 2.01（±0.03）g were randomly divided into four treatment groups with four replicates in each treatment group and 15 fishes in each replicate. Results showed that,compared with control group,weight gain rate,albumin level,lysozyme activity,and levels of immunoglobulin M and complement 3 in serum significantly increased by different types of antimicrobial lipopeptides supplementation,the levels of urea nitrogen,total cholesterol,triglyceride and low density lipoprotein cholesterol in serum significantly decreased. The high density lipoprotein cholesterol level,glutamic-oxalacetic transaminease activity and glutamic-pyruvic transaminase activity were not affected by different types of antimicrobial lipopeptides supplementation. The above parameters of antimicrobial lipopeptides supplementation groups were similar. In conclusion,different types of antimicrobial lipopeptides might have the similar effect on growth promotion and improvement of serum biochemical indexes and non-specific immune ability of GIFT tilapia under present experimental condition.