摘要
利用明胶与海藻酸钠协同固定葡萄糖氧化酶(GOD),研究固定化酶对葡萄糖的催化反应.结果表明:在海藻酸钠质量浓度为2%,海藻酸钠与明胶配比是8∶1,氯化钙质量浓度是6%,酶用量为1.0%,固定化时间为30 min,固定化温度为35℃,固定化p H为6.0,海藻酸钠与明胶协同固定化GOD的活力回收率为85.76%;固定化GOD重复使用7次后,酶活力回收率仍保持在32%以上,显示出良好的操作稳定性.
In order to investigate the catalytic reaction of immobilized enzyme to glucose, we utilized sodium alginate and gelatin to co-immobilize glucose oxidase （GOD）. The results showed that： the activity recovery of GOD, which co-immobilized by sodium alginate and gelatin, could reach 85.76% when the mass concentration of sodium alginate was 2%, the ratio of sodium alginate to gelatin was 8 ： 1, the mass concentration of calcium chloride was 6%, the dosage of enzyme was 1%, the time of immobilization was 30 min, the temperature of immobilization was 28℃, the pH of immobilization was 6.0. Furthermore, the enzyme activity recovery remained above 32% even after the immobilized GOD reused more than 7 times, which exhibited a good operational stability.
出处
《河南科学》
2015年第12期2111-2116,共6页
Henan Science
基金
河南省高等学校重点科研项目(15A180005)
