肝移植术后稳定生存者外周血中差异表达miRNA及其功能预测

Differential expressions of microRNAs and their predicted targets in liver transplant recipients with long-term stable survival

目的寻找肝移植术后长期稳定生存者外周血中有意义的生物学标志物。方法纳入肝移植术后稳定存活患者29例(STA组),反复排斥反应者10例(RJ组),健康对照者17例(HC组),利用芯片检测各组PBMCs中micro RNAs的表达谱,并用Real-time PCR对差异表达的micro RNAs进行验证。结果芯片结果显示STA组对比于RJ组共13个micro RNA表达明显下调,经Real-time PCR验证3个micro RNA(mi R-106b,mi R-18b,mi R-340)表达下调。结论肝移植患者外周血mi R-106b、mi R-18b、mi R-340可作为肝移植术后潜在标志物。

Objective To determine whether the mi RNA expression profile in peripheral blood mononuclear cells（PBMCs）differs between liver transplant recipients with long-term stable survival and those with acute rejection. Methods Twenty-nine liver transplant recipients with long-term stable survival（STA） group, 10 recipients with acute rejection（RJ group）, and 17 healthy subjects（control group） were recruited for genome-wide microarray analysis of mi RNA expressions in the PBMCs.The differentially expressed mi RNAs among the 3 groups were validated by real-time PCR, and the targets of these mi RNAs were predicted. Results Compared with the RJ group, the STA group showed down-regulation of 13 mi RNAs in the PBMCs.Of these down-regulated mi RNAs, mi RNA-18 b, mi RNA-340 and mi RNA-106 b were validated by real-time PCR, and the latter two mi RNAs were predicted to target the TGF-β pathway. Conclusions The differentially expressed mi RNAs in liver transplant recipients with long-term stable survival, namely mi RNA-18 b, mi RNA-340 and mi RNA-106 b, can be potential clinical biomarkers to predict the outcomes of liver transplantation.