WWOX和CD133在结直肠癌中的表达及临床意义

Expressions of WWOX and CD133 in colorectal cancer and their clinical significance

目的检测结直肠癌(CRC)组织中WWOX和CD133蛋白的表达情况及其相互之间的关系,并探讨它们与CRC患者临床病理参数之间的关系。方法采用免疫组织化学Elivision TM plus法检测174例CRC组织和80例正常结直肠黏膜组织中WWOX和CD133蛋白的表达情况。结果在CRC组织中WWOX和CD133蛋白表达的阳性率分别为41.4%和53.4%;对照组中WWOX和CD133蛋白表达的阳性率分别为87.5%和5.0%,其表达差异在两组之间有统计学意义(P<0.05)。WWOX和CD133蛋白表达与CRC患者肿瘤组织的不同分化程度、浸润深度、淋巴结是否转移以及不同Duke分期等之间差异均有统计学意义(P<0.05),且WWOX蛋白表达的阳性率与CD133蛋白的阳性率之间呈负相关关系(P<0.05)。Kaplan-Meier法生存分析结果显示在CRC组织中WWOX蛋白阳性表达组患者术后总的存活时间明显高于其阴性组患者,而CD133蛋白阳性组患者术后总的存活时间明显低于其阴性组患者,log-rank单因素分析差异有统计学意义。Cox多因素分析显示WWOX和CD133蛋白的阳性表达及Duke分期是影响CRC患者术后生存的独立预后因子。结论反常表达的WWOX和CD133参与了CRC的发生、发展、侵袭以及转移;在CRC患者中联合检测WWOX和CD133蛋白的表达对预测其进展和预后均有重要意义。

Objective To investigate the expressions of WWOX and CD133 in colorectal cancer（CRC） and their relationship with the clinicopathologic characteristics of CRC. Methods The expressions of WWOX and CD133 proteins were examined by immunohistochemistry in 174 specimens of CRC tissues and 80 normal colorectal mucosa tissues. Results The positivity rates of WWOX and CD133 proteins were 41.4% and 53.4% in CRC tissues, respectively, significantly different from the rates in normal colorectal mucosa tissues（87.5% and 5.0%, respectively; P〈0.05）. WWOX and CD133 protein expressions were significantly correlated with the histological grades of the tumors, depth of invasion, lymph node metastasis, and Duke＇s stages（P〈0.05）. Spearman analysis showed a negative relationship between the WWOX expression and CD133 expression（P〈0.05）.Kaplan-Meier survival analysis showed that the overall survival time of CRC patients with a positive expression of WWOX was longer than that of patients with a negative expression of WWOX; the overall survival time of patients with a positive expression of CD133 was shorter than that of the negative patients（P〈0.05）. COX regression analysis identified positive expressions of WWOX and CD133 protein and Duke＇s stage as the independent prognostic factors of CRC. Conclusion Abnormal expressions of WWOX and CD133 might be involved in the initiation, development, invasion, and metastasis of CRC. A combined detection of WWOX and CD133 can help in predicting the progression and prognosis of CRC.