应用PCR-RDB杂交技术检测人类乳头瘤病毒基因型的临床意义

Clinical significance of genotyping detection of human papillomavirus by using PCR-RDB

目的采用聚合酶链反应(PCR)和反向斑点杂交(RDB)技术检测西安地区人类乳头瘤病毒(HPV)感染流行状态和HPV基因型分布特征。方法共收集266例脱落细胞标本,其中尖锐湿疣86例、宫颈上皮内瘤变144例、宫颈癌36例,应用PCR-RDB杂交技术进行23种HPV基因型分型检测。结果全部标本HPV总阳性率为71.43%(190/266),宫颈癌HPV阳性率明显高于其他疾病,差异有统计学意义(χ2=6.297,P<0.05);HPV阳性标本基因分型全部成功,HPV16型为主要感染基因型,其次为HPV18、6、11型;单基因型总感染率91.58%(174/190),多基因型总感染率8.42%(16/190)。不同疾病单基因型感染和多基因型感染构成比差异无统计意义(χ2=0.3511,P>0.05)。结论 HPV16型是西安地区主要感染基因型,应用PCR-RBD技术检测HPV基因型对HPV感染的防治具有一定临床意义。

Objective To study the HPV infection situation and its genotype distribution characteristics in Xi′an by using polymerase chain reaction（PCR）and reverse dot blot（RDB）technology.Methods 266 samples of exfoliative cells were collected,including 86 cases of condyloma acuminatum,144 cases of cervical intraepithelial neoplasia（CIN）and 36 cases of cervical cancer.23 kinds of HPV genotyping detection were performed by using PCR-RDB.Results The total positive rate of HPV in all of the samples was 71.43%（190/266）.The HPV positive cases in cervical cancer was significantly higher than that in the other diseases（χ2=6.297,P〈0.05）.Genotyping in the HPV positive samples was successfully conducted.HPV16 was the most predominant genotype,the next genotypes were HPV18,6and 11.The overall infection rate of single HPV genotype was 91.58%（174/190）,which of multiple HPV genotypes was 8.42%（16/190）.There was no statistically significant difference of the constituent ratio between single genotype infection and multiple genotype infection in different diseases（χ2=0.351 1,P〉0.05）.ConclusionHPV16 is the main infection genotype in Xi′an area.Detection of HPV genotypes by using PCR-RDB technology has certain significance to the prevention and treatment of HPV infection.