以腐烂水果为营养源高效制备细菌纤维素

Cost-efficient Production of Bacterial Cellulose by Gluconacetobacter xylinus using Rotten Fruits as the Culture Medium

细菌纤维素(Bacterial Cellulose,简称BC)是一种由微生物合成的天然纤维素材料。本文选取腐烂水果,如苹果、西瓜、桃子、梨为廉价营养源高效制备细菌纤维素。研究了培养基灭菌方法以及添加氮源等因素对糖转化率、BC干重以及产量的影响。结果表明,单独以腐烂水果汁为营养源所得BC产量高于目前普遍使用的葡萄糖培养基。另外,过滤灭菌和添加氮源有助于获得较高的BC产量。

Bacterial cellulose （BC） is a kind of natural cellulose synthesized by microbial fermentation. In this work, some rotten fruits, e.g. apple, watermelon, peach and pear, were utilized as the fermentation nutrient sources for cost-efficient and mass production of BC. Different sterilization processes of the rotten fruit juices including filtration and autoclaving, and two kinds of culture medium, i.e. with or without supplement of nitrogen sources were tried, and their effects on sugar conversion ratio, BC dry weight and BC production yield were investigated, using glucose for comparison. It was found that the BC production yields from the sole rotten fruit juices were higher than that by glucose with nitrogen sources. Additionally filter-sterilization of the juices and supplement of nitrogen sources was helpful to obtain a high production yield, with the rotten peach showing the highest value of 17.47% among all test fruits under these conditions.