薄层色谱法评价不同产地黄根的品质

Identification of Prismatomeris connate Y.Z.Ruan from Different Origin by TLC

【目的】建立评价黄根(Prismatomeris connate Y.Z.Ruan)品质的薄层色谱分析方法。【方法】以有效成分甲基异茜草素为指标,以甲苯-乙酸乙酯-甲醇-甲酸(3∶1∶0.2∶0.1)为展开剂,建立黄根的专属性薄层色谱,并评价10个不同产地黄根的品质。【结果】不同产地黄根药材与对照色谱在相应位置上,显示出相同颜色的斑点,且斑点清晰,比移值(Rf)为0.76。【结论】薄层色谱法能够有效对黄根进行鉴别,且该方法简便、准确,具有较好的适应性。

【Objective】To establish a TLC method for the identification of Prismatomeris connate Y.Z.Ruan.【Methods】Rubiadin was indicators,methylbenzene-ethyl acetate-methanol-formic acid(3∶1∶0.2∶0.1)was developing agent.【Results】The same color spots were shown in the corresponding position of the Prismatomeris connate Y.Z.Ruan from different origin.The spots were clear and the Rf index was 0.76.【Conclusion】The TLC method is effective for the identification of the Prismatomeris connate Y.Z.Ruan.The method is simple and reliable.