薄层色谱法评价不同产地土茯苓的品质

Identification of Smilax glabra Roxb.from Different Origin by TLC

【目的】建立评价土茯苓(Smilax glabra Roxb.)品质的薄层色谱分析方法。【方法】以有效成分落新妇苷为指标,以氯仿-甲醇-甲酸(4∶1∶0.05)展开系统,建立土茯苓的专属性薄层色谱,并评价10个不同产地土茯苓的品质。【结果】不同产地土茯苓药材在与对照色谱相应位置上,显示出相同颜色的荧光斑点,且斑点清晰,比移值(Rf)为0.41。【结论】薄层色谱法能够有效对土茯苓进行鉴别,且该方法简便、准确,具有较好的适应性。

【Objective】To establish a TLC method for the identification of Smilax glabraRoxb..【Methods】Astilbin used as indicators,chloroform-methanol-formic acid(4∶1∶0.05)used as developing agent.【Results】The same color spots were shown in the corresponding position of the Smilax glabra Roxb.from different origin,and the spots were clear.The Rf index was 0.41.【Conclusion】The TLC method is effective for the identification of the Smilax glabra Roxb.,which is simple and reliable.