慢性乙型肝炎患者CD_8^+ T细胞TCR-Vβ基因CDR3区克隆化特征的研究

Study on cloning characteristic of chronic hepatitis B patients' CD_8^+ T cell receptor-Vβ gene CDR3

目的:检测慢性乙型肝炎(CHB)患者外周血T细胞TCR-Vβ基因CDR3的克隆化特征。方法:选择20例慢性乙型肝炎患者设为观察组;选择20例健康体检者设为对照组,采用多引物巢式PCR法两步扩增22个TCR-Vβ基因亚家族,高分辨率琼脂糖凝胶电泳法定性检测两组研究对象的PCR产物的克隆化特征。结果:CHB患者CD_8^+T细胞TCR-Vβ1、Vβ3、Vβ9、Vβ14出现优势增生。结论:CHB患者外周血CD_8^+T细胞Vβ1、Vβ3、Vβ9、Vβ14存在克隆性增生。

Objective: To detect cloning characteristic of T cell receptor- Vβ gene CDR3 appeared in peripheral blood of patients with the chronic hepatitis B. Methods: Twenty patients with chronic hepatitis B and twenty healthy adults were selected. 22TCR- Vβ gene subfamilies were amplified by multi- primer nested PCR two- step amplification method,and the cloning characteristic of PCR products were qualitatively detected by high resolution agarose gel electrophoresis. Results: Dominant hyperplasia situs in CD_8^+T cells were Vβ1,Vβ3,Vβ9,and Vβ14. Conclusions: TCR- Vβ1,Vβ3,Vβ9,and Vβ14 of CD_8^+T cells in the peripheral blood of the CHB patients exist conspicuous clonal proliferation.