糖尿病合并肺结核耐多药快速检测

Rapid detection of multi-drug resistane of Pulmonary tuberculosis complicated with diabetes

目的探讨DNA微阵列芯片法快速检测糖尿病合并肺结核耐多药结核分枝杆菌基因型的临床应用价值。方法应用DNA微阵列芯片法对197份单纯肺结核和163份糖尿病合并肺结核涂阳痰液样本进行kat G、inh A、rpo B耐药基因突变位点检测,并分别与传统药敏试验结果比较。结果 DNA微阵列芯片法对单纯肺结核耐多药检出率为42.6%(84/197),对糖尿病合并肺结核耐多药检出率为53.4%(87/163),差异有显著性(P<0.05);比较传统药物敏感性试验和DNA微阵列芯片两种方法分别检测单纯肺结核组结核分枝杆菌耐多药率、糖尿病合并肺结核组结核分枝杆菌耐多药率,差异无显著性(P>0.5)。结论应用DNA微阵列芯片法快速检测糖尿病合并肺结核耐多药基因型,快速可行,可作为传统药敏试验的补充。

Objective To explore the clinical application value of detecting multi-drug resistance Pulmonary tuberculosis complicated with diabetes. Method The drug-resistant genes of kat G、inh A、rpo B were detected in the positive sputum specimens by acid fast stain of 197 Pulmonary tuberculosis and 163 Pulmonary tuberculosis complicated with diabetes by DNA microarray genechip,the results were compared with that of traditional drug susceptibility test（ DST）. Result The drug-resistant rate were 42. 6%（ 84 /197） in Pulmonary tuberculosis and 53. 4%（ 87 /163） in Pulmonary tuberculosis complicated with diabetes by DNA microarray genechip,there was significant difference;compared the traditional drug susceptibility test and DNA microarray genechip,the drug-resistant rates of mycobacterium tuberculosis in Pulmonary tuberculosis and in Pulmonary tuberculosis complicated with diabetes were no significant difference sitatistically. Conclusion DNA microarray genechip can rapidly and feasibly detect multi-drug resistant genetype of Pulmonary tuberculosis complicated with diabetes,and can be used to supplement of traditional drug susceptibility test.