甜茶素对棕榈酸诱导INS-1细胞超微结构及CytC易位表达的保护作用

Protective Effects of Rubusoside on the Ultrastructure and Cyt C Translocation Expression of the Palmitic Acid- induced INS- 1 Cells

目的:探索甜茶素对棕榈酸诱导INS-1细胞损伤的保护机制。方法:MTT法检测正常对照组、棕榈酸模型组、不同浓度甜茶素组细胞存活率,透射电镜观察各组细胞超微结构,免疫电镜观察各组细胞色素C的易位表达。结果:不同浓度甜茶素对棕榈酸诱导的INS-1细胞具有保护作用,超微结构保存较好;模型组细胞凋亡增多且超微结构损伤严重,Cyt C蛋白从线粒体释放入细胞质。结论:甜茶素可以提高棕榈酸诱导INS-1损伤的细胞存活率,抑制细胞凋亡的发生,其机制可能与抑制棕榈酸引起的氧化应激和阻碍Cyt C从线粒体转位入细胞质,激活细胞凋亡蛋白酶级联反应有关。

ObjectiveTo investigate the protective mechanism of Rubusoside treating on the palmitic acid - induced INS - 1 cells. Methods MTI＂ assay was used to detect the cell viability rate of control group, palmitic acid group and different concentrations of Rubusoside group. Transmission electron microscope （TEM） was used to observe the uhrastructur of every group and the immune electron microscopy was used to detect cytochrome C translocationexpression. Results As evidenced by M33＂ assay, the cell viabihty signifi- cantly was enhanced by different concentrations Rubusoside pretreatment. TEM revealed degenerative changes in the ultramicroscopic structure of palmitic acid - induced INS - 1 cells whereas various concentrationpretreatment with Rubusoside could significantly attenuated the deterioration of the damage. Immune electron microscopy showed that different concentrations Rubusoside could prevent the cy- tochrome C releasing from mitochondria to cytoplasm with palmitic acid induced. Conclusion Rubusoside can improve the survival rate of palmitic acid - induced INS - 1 cells and inhibit the occurrence of apoptosis. It is suggested that the mechanism may be associated with the inhibition of the oxidative stress induced by palmitic acid and prevent the cytochrome C releasing from mitochondrial to cytoplasm, which can activate caspase cascade and a downstream signaling to apopotosis.