n-3脂肪酸对重症急性胰腺炎早期调控机制研究

Research of n-3 fatty acid for early regulatory mechanism in severe acute pancreatitis

目的探讨n-3脂肪酸对大鼠重症急性胰腺炎(SAP)早期调控机制。方法 30只SD大鼠随机分为正常组(开腹后翻动胰腺数次后尾静脉注射生理盐水)、治疗组(造模成功后,尾静脉注射n-3脂肪酸)和SAP组(造膜成功后,尾静脉注射生理盐水),各10例,并3 h取材。观察血清丙二醛(MDA)、淀粉酶(AMY)、超氧化物歧化酶(SOD),胰腺核因子κB(NF-κB)及病理损伤。结果 SAP组血清MDA、AMY、胰腺NF-κB及病理损伤明显高于治疗组和正常组(P<0.05),治疗组这些指标均高于正常组(P<0.05);而SOD在正常组明显高于治疗组和SAP组(P<0.05),治疗组明显高于SAP组(P<0.05)。结论 n-3脂肪酸是一种免疫调理营养脂肪乳剂,早期具有调节炎症反应,减少氧自由基生产,减轻胰腺组织损伤,对胰腺炎具有一定保护作用。

Objective To investigate n-3 fatty acid for early regulatory mechanism in severe acute pancreatitis（SAP）. Methods A total of 30 SD rats were randomly divided into normal group（normal saline by tail intravenous injection after pancreas flipped for several time in laparotomy）, treatment group（n-3 fatty acid by tail intravenous injection after molding） and SAP group（normal saline by tail intravenous injection after molding）, with 10 cases in each group. Observations were made on serum methane dicarboxylic aldehyde（MDA）, amylase（AMY）, superoxide dismutase（SOD）, pancreas nuclear factor κB（NF-κB）, and pathological damage. Results SAP group had obviously higher serum MDA, AMY, pancreas NF-κB, and pathological damage than treatment group and normal group（P〈0.05）. These indexes were also higher in treatment group than in normal group（P〈0.05）. Normal group had much higher SOD than treatment group and SAP group（P〈0.05）. Treatment group had much higher SOD than SAP group（P〈0.05）. Conclusion As a nutrition fat emulsion for immune regulation, n-3 fatty acid can regulate early inflammatory reaction, reduce occurrence oxygen radical, and decrease pancreatic tissue damage. It also contains certain protective effect for pancreatitis.