HBV感染的原发性肝癌患者外周血清IL-12,IL-17水平检测及临床意义

The Detection and Clinical Significance of Serum Levels of IL-12,IL-17 in Primary Liver Cancer Patients Infected by HBV

目的探究乙型肝炎病毒(HBV)感染后并发原发性肝癌患者外周血清白介素-12(IL-12)、白介素-17(IL-17)水平检测及临床意义。方法将112例感染HBV患者分为乙型肝炎肝硬化组、原发性肝癌无远处侵犯组和原发性肝癌远处侵犯组(包括门静脉侵犯)3组,同时以健康查体人群作为对照组。采用双抗体夹心法酶联免疫吸附试验(ELISA)测定不同组人群外周血清IL-12,IL-17的含量,并进行统计学分析。结果乙型肝炎肝硬化组、原发性肝癌组与对照组比较,IL-17增高,IL-12降低,差异具有统计学意义(P<0.05)。原发性肝癌两组之间比较,IL-17继续增高,IL-12仍降低,差异具有统计学意义(P<0.05)。结论 IL-12,IL-17参与了HBV感染所致肝硬化及原发性肝癌的发病及病情进展的过程,为原发性肝癌患者治疗效果的评价提供实验依据。

Objective To research the expression levels and clinical significance of serum levels of interleu-kin-12（IL-12）,interleukin-17（IL-17） in primary liver cancer patients infected by HBV.Methods 112 patients infec-ted by HBV were divided into 3 groups：hepatitis B cirrhosis group,primary liver cancer without distant invasion,and pri-mary liver cancer with the distant invasion group（ including portal vein invasion） ,at the same time,healthy people were taken as a control group.The contents of IL-17 and IL-12 in peripheral blood of different groups were measured by en-zyme-linked immunosorbent assay（ ELISA） .Results IL-17 was higher and IL-12 was lower and the difference was sta-tistically significant（P〈0.05）.Compared with the 2 groups of primary liver cancer,with the progress of the tumor,IL-17 continued to increase,IL-12 was still lower,the difference was statistically significant（P〈0.05）.Conclusion IL-12 and IL-17 are involved in the pathogenesis and progression of liver cirrhosis and primary liver cancer caused by HBV in-fection.This study provided an experimental basis for the evaluation of the treatment effect of the patients with primary liver cancer.