人类疱疹病毒6A亚型潜伏感染人神经胶质瘤细胞系U251

Human herpesvirus 6A subtype latent infection of human glioma cell line U251

目的 :采用人神经胶质瘤细胞系U251作为宿主建立HHV-6A亚型潜伏感染神经系统细胞的体外模型。方法:HHV-6A急性感染U251细胞后随细胞传代,通过观察细胞形态变化、病毒复制及检测病毒基因表达情况等确定其进入潜伏感染阶段。结果:HHV-6A在急性感染U251细胞后随传代数的增加,病毒基因拷贝量逐渐减低至稳定的低水平,而代表处于潜伏感染阶段的HHV-6A病毒U94基因的转录逐渐下降随后维持在稳定水平表达。结论:HHV-6A在体外感染U251后通过传代使病毒从急性感染期逐渐转入潜伏感染期。

Objective：To establish the model of human herpesvirus（HHV）-6A subtype latent infection of human glioma cell line U251 in vitro. Methods： U251 cells infected by HHV-6A were cultured and passaged for model establishment. The cell morphology changing, virus replication and viral gene expression were detected to identify the latent infection stage. Results： With increase of HHV-6A infection of U251 cells algebra, the viral gene copy number was gradually decreased to a stable low level. The HHV-6A U94 gene, a representative of the latent stage of infection of the virus, was gradually decreased and then maintained at a stable level. Conclusion：HHV-6A was successfully transferred from the acute infection stage to the latent infection stage in U251 cells in vitro.