非增殖性糖尿病视网膜病变患者血清miR-29c的表达及相关分析

Expression of serum miR-29c and its correlation analysis in patients with non-proliferative diabetic retinopathy

目的 :观察微小RNA-29c(miR-29c)在非增殖性糖尿病视网膜病变(non-proliferative diabetic retinopathy,NPDR)患者血清中的表达水平,探讨其在糖尿病视网膜病变(diabetic retinopathy,DR)发病机制中的作用。方法:选择2型糖尿病NPDR患者及无视网膜病变(non-diabetic retinopathy,NDR)患者各30例。收集血清样本,应用荧光定量PCR方法检测血清miR-29c的相对表达水平。应用Pic Tar、Target Scan与Mi Randa软件综合预测miR-29c的靶基因,取三款软件的交集作为最终靶基因。结果:miR-29c在NPDR患者血清中的表达量明显高于NDR组(P<0.05),其表达量与Hb A1c呈显著正相关(r=0.379,P<0.05)。预测miR-29c靶基因集合富集在磷酸肌醇代谢、细胞因子及受体的相互作用、细胞外基质与受体信号作用通路中。结论:NPDR患者血清miR-29c表达显著上调,miR-29c可能通过调控细胞因子的相互作用、磷酸肌醇代谢等途径参与DR的发生发展。

Objective：To investigate the expression of serum miR-29 c in the patients with non-proliferating diabetic retinopathy（NPDR） and explore the role of miR-29 c in diabetic retinopathy（DR）. Methods： Patients with NPDR（n=30） and without DR（n=30） were enrolled. Total RNAs including miRNA was extracted from serum samples. The levels of miR-29 c were detected by quantitative Real-time PCR. Pic Tar, Target Scan and Mi Randa were performed to comprehensively predict target genes of miR-29 c,and the intersection of the three softwares was set as the final target genes. Results： The expression of serum miR-29 c was significantly elevated in the NPDR group than that in the NDR group（P 〈0.05）. Positive correlation between miR-29 c expression and Hb A1 c level（r =0.379, P 〈0.05） was observed. The target genes of miR-29 c were significantly enriched in inositol phosphate metabolism, interaction between cytokine and cytokine receptor, and ECM and receptor. Conclusion： The serum levels of miR-29 c were significantly increased in the NPDR group. Mi R-29 c may play an important role in pathogenesis of DR by regulating cytokines interaction and inositol phosphate metabolism.