植入式闭环芯片系统对清醒兔心率的调控作用

Effect of a closed-loop implanted chip system on heart rate regulation in conscious rabbits

目的 :通过电刺激迷走神经观察植入式闭环芯片系统对清醒兔心率的调控作用。方法 :将12只心率正常的新西兰兔随机分2组:对照组、迷走神经电刺激(vagus nerve stimulation,VNS)组。VNS组兔体内植入自主研发的闭环芯片1周,随机电刺激右侧迷走神经1 h,恢复1 h;对照组体内植入无刺激的芯片。MD2000WL软件记录实时心电图(electrocardiogram,ECG),芯片根据ECG计算心率。结果:植入式闭环芯片可在清醒兔体内工作。芯片可以成功记录完整心电图。VNS组调控期间心率明显低于调控前的基础心率[(265.2±15.2)次/min vs.(289.5±15.7)次/min,P<0.05]。VNS组调控期间心率和对照组相比较,VNS组的心率明显降低[(-24.3±3.6)次/min vs.(-4.6±5.1)次/min,P<0.05]。结论:植入式闭环芯片系统可以长期调控清醒兔的心率。

Objective： To assess the effects of a closed-loop implanted chip system on heart rate regulation in conscious rabbits.Methods： Twelve rabbits with normal heart rate（HR） were randomly divided into the control and the vagus nerve stimulation（VNS） groups.In the VNS group, all the animals were implanted the self-developed closed-loop chip for a week. The right vagus nerve of rabbit was randomly stimulated by the chip according to real-time feedback of HR at different times. Each rabbit underwent 1 hour of regulation and 1hour of recovery. The control group was implanted the chip without any stimulation. MD2000 WL software recorded the real-time electrocardiogram（ECG）. HR was calculated by the chip according to the ECG. Results： The implanted closed-loop chip system worked well in conscious rabbits in vivo. The chip recorded complete ECG. There was a significant decrease of HR during the regulation [（265.2 ±15.2） bpm vs.（289.5 ± 15.7） bpm, P 0.05] compared with the baseline HR in the VNS group. The HR was also significantly decreased during regulation in the VNS group[（-24.3 ± 3.6） bpm vs.（-4.6 ± 5.1） bpm, P〈 0.05] compared with the control group. Conclusion： The implanted closed-loop chip system can successfully regulate the heart rate of conscious rabbits in long term.