FcγRI参与大鼠神经病理性疼痛的发生

FcγRIIS INVOLVED IN THE DEVELOPMENT OF NEUROPATHIC PAIN IN RATS

目的:研究脊髓中FcγRI介导的免疫反应在大鼠坐骨神经慢性压迫性损伤(chronic constriction injury of the sciatic nerve,CCI)模型中的作用。方法:成年雄性SD大鼠96只,随机分为:1空白对照组(C组):不进行任何处理;2假手术组(S组):只分离暴露坐骨神经不结扎;3神经病理性疼痛组(NP组):建立CCI模型;4 NP+FcγRI中和抗体组(NF组):建立CCI模型并在坐骨神经周围置管给予FcγRI中和抗体;5 Ig G免疫复合物组(IIC组):坐骨神经周围置管给予Ig G免疫复合物;6 IC+FcγRI中和抗体组(IF组):坐骨神经周围置管后给予Ig G免疫复合物、FcγRI中和抗体。各组大鼠分别术前和术后1、3、7、14、21 d测定后爪机械缩足阈值(paw withdrawal mechanical threshold,PWMT)和热缩足潜伏期(paw withdrawal thermal latency,PWTL),利用蛋白印记(western blotting)与荧光PCR技术检测大鼠脊髓FcγRI蛋白和m RNA表达量的变化。结果:NP组和IIC组可引起大鼠术侧后爪机械刺激和热刺激痛觉过敏,FcγRI蛋白和m RNA表达量明显上调(P<0.05)。给予中和抗体后可明显减轻大鼠机械痛和热痛,FcγRI蛋白和m RNA表达量明显降低(P<0.05)。结论:FcγRI介导的免疫反应参与大鼠神经病理性疼痛的发生。

Objective： To study the role of FcγRI-mediated neural immune response in spinal cord with chronic constriction injury（CCI） of the sciatic nerve. Methods： Ninety-six Sprague-Dawley rats were divided into six groups：1 Control group（group C）： without any treatment; 2 Sham operation group（group S）： exposed the sciatic nerve only;3 Neuropathic pain group（group NP）： CCI model; 4 NP＋FcγRI neutralizer group（group NF）： CCI model ＋ FcγRI neutralizer around the sciatic nerve; 5 Ig G group（group IIC） ： Ig G around the sciatic nerve; 6 IIC ＋FcγRI neutralizer group（group IF）： Ig G ＋ FcγRI neutralizer around the sciatic nerve. Mechanical and thermal nociceptive thresholds were assessed by measuring hind paw withdrawal to the von Frey filaments and radiant heat stimulation, respectively, one day prior to the operation（baseline） and 1, 3, 7, 14, and 21 days after operation. FcγRI protein and FcγRI m RNA of the spinal cord tissue was detected by western blotting and fluorescent polymerase chain reaction. Results： We found that CCI and IIC induced mechanical allodynia and thermal hyperalgesia in hind paws of operation side. Injection of the neutralizing antibody relieved mechanical and thermal hyperalgesia. Expression of FcγRI protein and FcγRI m RNA were upregulated in CCI group and IIC group（P〈 0.05）. Expression of FcγRI protein and FcγRI m RNA were reduced after treatment with FcγRI neutralizer. Conclusions： FcγRI mediated immune response may be involved in the development of neuropathic pain.