血清可溶性间皮素相关肽、糖链抗原125联合检测对腹膜恶性间皮瘤的诊断价值

Significance of combined detection of serum SMRP and CA125 in the diagnosis of malignant peritoneal mesothelioma

目的：探讨血清可溶性间皮素相关肽（SMRP）、糖链抗原125（CA125）联合检测对腹膜恶性间皮瘤的诊断价值。方法选择20例腹膜恶性间皮瘤患者作为恶性组，50例卵巢良性肿瘤患者作为良性组，60例健康妇女作为对照组。采用酶联免疫吸附法（ELISA）检测血清 SMRP 水平，电化学发光免疫法测定血清CA125水平。比较各组 SMRP、CA125水平，分析 SMRP、CA125诊断腹膜恶性间皮瘤的敏感性、特异性。结果恶性组 SMRP、CA125水平分别为（14．8±1．6）μg／L、（189．1±15．3）μg／L，显著高于良性组[分别为（3．9±0．9）μg／L、（28．6±4．2）μg／L]（t ＝27．40、49．610，均 P ＜0．01）及对照组[分别为（2．8±0．7）μg／L、（16．9±3．8）μg／L]（t ＝29．877、53．334，均 P ＜0．01）。SMRP、CA125单独检测诊断腹膜恶性间皮瘤的敏感性分别为65．0％、50．0％，特异性分别为83．6％、79．1％，SMRP、CA125联合检测诊断腹膜恶性间皮瘤的敏感性为95．0％，特异性为93．6％。结论SMRP 对腹膜恶性间皮瘤的诊断有一定的价值，SMRP、CA125联合检测可提高腹膜恶性间皮瘤诊断的敏感性和特异性。

Objective To evaluate the values of combined detection of soluble mesothelin related proteins （SMRP）and carbohydrate antigen 1 25 （CA1 25）in the diagnosis of malignant peritoneal mesothelioma.Methods 20 patients with malignant peritoneal mesothelioma were selected as the malignant group,50 patients with benign ovar-ian tumor were selected as the benign group,60 healthy women were selected as the control group.Enzyme -linked immunosorbent assay（ELISA）was adopted to detect serum SMRP levels and electrochemiluminescence immunifaction （ECLI）was adopted to detect serum CA1 25 levels.The SMRP,CA1 25 levels were compared among all groups,the sensitivity,specificity of SMRP and CA1 25 in the diagnosis of peritoneal malignant mesothelioma were analyzed. Results Serum levels of SMRP,CA1 25 in malignant group were （1 4.8 ±1 .6 ）μg/L,（1 89.1 ±1 5.3 ）μg/Lrespectively,which were significantly higher than those in the benign group[（3.9 ±0.9）μg/L,（28.6 ±4.2）μg/L] （t =27.40,49.61 0,all P 〈0.01 ）and the control group[（2.8 ±0.7）μg/L,（1 6.9 ±3.8）μg/L]（t =29.877, 53.334,all P 〈0.01 ）.The sensitivities of SMRP,CA1 25 detection alone for malignant peritoneal mesothelioma diag-nosis were 65.0%,50%,respectively,while their specificities were 83.6%,79.1 %.The sensitivity and specificity of combined detection of SMRP and CA1 25 for malignant peritoneal mesothelioma were 95.0% and 93.6%.Conclusion SMRP has some value in the diagnosis of peritoneal malignant mesothelioma.SMRP combined with CA1 25 detection can markedly improve the diagnostic sensitivity and specificity for malignant peritoneal mesothelioma.