电针丰隆穴对高脂血症大鼠肝脏SR-BI、PPARγ的调控及炎症相关因子的影响

Effect of electroacupuncture at Fenglong on hepatic scavenger receptor class B type I and peroxisome proliferator-activated receptor expression and inflammatory factors in rats with hyperlipidemia

目的:探讨电针丰隆穴对高脂血症大鼠血脂水平、肝脏组织的清道夫受体BI(scavenger receptor clas s B type I,SR-BI)、过氧化物酶体增殖物激活型受体γ(peroxi some proliferator-activated receptor-γPPARγ)的mRNA相对表达量及腹腔巨噬细胞炎症相关因子细胞间黏附因子-1(intercellular adhesion molecule-1,ICAM-1)、白介素-1β(intefleukin-1β,IL-1β)、IL-10含量的影响及其相关作用机制.方法:将SD大鼠随机分为空白对照组、模型对照组、电针1组、电针2组这4组,每组10只.治疗结束后检测各组血脂含量,肝脏组织中SR-BI、PPARγ的mRNA相对表达量.最后分离出大鼠腹腔中的巨噬细胞,应用流式细胞仪检测炎症相关因子ICAM-1、IL-1β、IL-10的含量.结果:与空白对照组比较,模型对照组TG.变化并不明显(p>0.05),而总胆固醇(total chole sterol,TC)、低密度脂蛋白-胆固醇(loW density lipoprotein cholesterol,LDL-C)明显上升(P<0.01),高密度脂蛋白-胆固醇(high density lipoprotein cholesterol,HDL-C).明显下降(P<0.01);与模型对照组比较,电.针l组及电针2组的TG和HDL-C变化并不明显(P>0.05),大鼠血清TC、LDL-C均有显著下降(P<0.01);与电针1组比较,电针2组TC、LDL-C均有降低(P<0.01,P<0.05).与空白对照组比较,除了电针2组外,其余两组SR-BI、PPAR7均有显著降低(P<0.05,P<0.01);与模型对照组比较,电针1组和电针2组的SR-BI、PPARγ均有显著升高(P<0.05,P<0.01),其中电针2组更为显著(P<0.01).与空白对照组比较,除电针2组外,模型对照组和电针1组的炎症因子ICAM-1、IL-1β均有显著性升高(P<0.05,P<0.01),而模型对照组的抗炎因子IL-10有显著下降(P<0.01);与模型对照组比较,电针1组和电针2组的炎症因子ICAM-1、IL-1β均有非常显著性减少(P<0.01),而抗炎因子IL-10的含量有非常显著性升高(p<0.01).结论:通过电针丰隆穴治疗能降低高脂血症模型大鼠的TC、LDL-C水平,升高HDL-C水平,增加肝脏SR-BI、PPARγmRNA相对表达量,减少大鼠巨噬细胞内炎症因子ICAM-1、IL-1β并增加抗炎因子IL-10的含量,达到治疗高脂血症的目的.

AIM：To investigate the effect of electroacupuncture（EA） at Fenglong on blood lipids,mRNA expression of scavenger receptor class B type I（SR-BI） and peroxisome proliferatoractivated receptor γ（PPARγ） in the liver,and inflammatory factors in a rat model of hyperlipemia.METHODS：Forty SD rats were randomly and equally divided into four groups：a blank control group（GA）,a model group（GB）,and two electroacupuncture groups（GC and GD）.After treatment,blood lipids were determined.The mRNA expressional levels of SR-BI and PPARy in the liver were detected by RTPCR.Next,the macrophages were isolated.Flow cytometry（FCM） was applied to test the expression of intercellular cell adhesion molecule-1（ICAM-1）,interleukin（IL）-Iβ and IL-10.RESULTS：Compared with GA,total cholesterol（TC） and low density lipoprotein cholesterol（LDL-C） were significantly higher and high density lipoprotein cholesterol（HDL-C） was significantly lower in GB（P〈0.01）.Compared with GB,the contents of TC and LDL-C were significantly decreased in GC and GD（P〈0.01）.Compared with GC,the contents of TC and LDL-C were significantly decreased in GD（P〈0.05 and P〈0.01）.Compared with GA,SR-BI and PPARγ were significantly decreased in GB and GC（P〈0.05 and P〈0.01）.Compared with GB,SRBI and PPARγ were significantly increased in GC and GD（P〈0.05 and P〈0.01）.Compared with GA,ICAM-1 and IL-1β were significantly increased in GB and GC（P〈0.05 and P〈0.01）,while anti-inflammatory factor IL-10 decreased significantly（P〈0.01）.Compared with GB,ICAM-1 and IL-1β were significantly increased in GC and GD（P〈0.01）,while anti-inflammatory factor IL-10 was increased significantly（P〈0.01）.CONCLUSION：Electroacupuncture at Fenglong can decrease TC and LDL-C,increase HDL-C and the mRNA expression of SR-BI and PPARy in the liver,reduce the contents of ICAM-1 and IL-1β,but increase the content of IL-10,thus achieving a therapeutic effect on hyperlipidemia.